Leishmaniasis in cat shelters: A serological, molecular and entomological study

Página do item simplificado
dc.contributor.authorLeonel, João Augusto Franco
dc.contributor.authorVioti, Geovanna
dc.contributor.authorAlves, Maria Luana
dc.contributor.authorBenassi, Julia Cristina
dc.contributor.authorSilva, Diogo Tiago da
dc.contributor.authorSpada, Julio Cesar Pereira
dc.contributor.authorRuiz, Vera Letticie de Azevedo
dc.contributor.authorStarke-Buzetti, Wilma Aparecida [UNESP]
dc.contributor.authorSoares, Rodrigo Martins
dc.contributor.authorOliveira, Trícia Maria Ferreira de Sousa
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-12T01:18:41Z
dc.date.available2020-12-12T01:18:41Z
dc.date.issued2020-09-01
dc.description.abstractAn epidemiological Leishmania spp. and entomological Phlebotomine sandflies survey was performed in cat shelters at leishmaniasis endemic area of Brazil. Blood and conjunctival swab (CS) samples were collected from 94 cats in two animal protection shelters. These samples were subjected to serological tests using the indirect immunofluorescence antibody test (IFAT) and indirect enzyme-linked immunosorbent assay (ELISA) and to molecular test by polymerase chain reaction (PCR). In addition, a Phlebotomine sandflies survey was performed in the same shelters. The analyses revealed a positivity of 31.91% (30/94) through ELISA and 29.79% (28/94) through IFAT. The two serological tests showed a positive association with perfect agreement (k = 0.925). None of the cats were positive by Leishmania spp. DNA. One Lutzomyia (Lutzomyia) longipalpis male was found in one of the cat shelters. The results and the implications of our findings are discussed below.en
dc.description.affiliationLaboratory of Applied Preventive Veterinary Medicine Department of Veterinary Medicine Faculty of Animal Science and Food Engineering University of São Paulo – USP
dc.description.affiliationPost-graduate program in Experimental Epidemiology Applied to Zoonoses Department of Preventive Veterinary Medicine and Animal Health Faculty of Veterinary Medicine and Animal Science University of São Paulo – USP
dc.description.affiliationDepartment of Biology and Animal Science School of Engineering São Paulo State University – UNESP
dc.description.affiliationDepartment of Preventive Veterinary Medicine and Animal Health Faculty of Veterinary Medicine and Animal Science University of São Paulo – USP
dc.description.affiliationUnespDepartment of Biology and Animal Science School of Engineering São Paulo State University – UNESP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2014/02882-3
dc.format.extent2013-2019
dc.identifierhttp://dx.doi.org/10.1111/tbed.13544
dc.identifier.citationTransboundary and Emerging Diseases, v. 67, n. 5, p. 2013-2019, 2020.
dc.identifier.doi10.1111/tbed.13544
dc.identifier.issn1865-1682
dc.identifier.issn1865-1674
dc.identifier.scopus2-s2.0-85082107995
dc.identifier.urihttp://hdl.handle.net/11449/198657
dc.language.isoeng
dc.relation.ispartofTransboundary and Emerging Diseases
dc.sourceScopus
dc.subjectFelis catus
dc.subjectLeishmania spp.
dc.subjectLutzomyia longipalpis
dc.subjectPCR
dc.subjectserology
dc.titleLeishmaniasis in cat shelters: A serological, molecular and entomological studyen
dc.typeArtigo
unesp.author.orcid0000-0003-0617-301X[1]
unesp.author.orcid0000-0003-3916-3600[10]

Arquivos

Coleções

Artigos