A new polymerase chain reaction/restriction fragment length polymorphism protocol for Plasmodium vivax circumsporozoite protein genotype (VK210, VK247, and P-vivax-like) determination

Nenhuma Miniatura disponível
Data
2007-12-01
Autores
Alves, Renata Tome [UNESP]
Povoa, Marinete Marins
Goldman, Ira F.
Cavasini, Carlos Eugnio
Baptista Rossit, Andrea Regina
Dantas Machado, Ricardo Luiz
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Resumo
For the molecular diagnosis of Plasmodium vivax variants (VK210, VK247, and P. vivax-like) using DNA amplification procedures in the laboratory, the choice of rapid and inexpensive identification products of the 3 different genotypes is an important prerequisite. We report here the standardization of a new polymerase chain reaction/restriction fragment length polymorphism technique to identify the 3 described P. vivax circumsporozoite protein (CSP) variants using amplification of the central immunodominant region of the CSP gene of this protozoan. The simplicity, specificity, and sensitivity of the system described here is important to determine the prevalence and the distribution of infection with these P. vivax genotypes in endemic and nonendemic malaria areas, enabling a better understanding of their phylogeny. (c) 2007 Published by Elsevier B.V.
Descrição
Palavras-chave
Plasmodium vivax genotypes, PCR/RFLP, diagnosis, circumsporozoite protein, Plasmodium vivax-like, VK210, VK247
Como citar
Diagnostic Microbiology and Infectious Disease. New York: Elsevier B.V., v. 59, n. 4, p. 415-419, 2007.
Coleções