AN IMPROVED CULTURE-MEDIUM FOR DETECTING LIVE YEAST PHASE CELLS OF PARACOCCIDIOIDES-BRASILIENSIS

dc.contributor.authorKurita, N.
dc.contributor.authorSano, A.
dc.contributor.authorCoelho, Kunie Iabuki Rabello [UNESP]
dc.contributor.authorTakeo, K.
dc.contributor.authorNishimura, K.
dc.contributor.authorMiyaji, M.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:28:20Z
dc.date.available2014-05-20T15:28:20Z
dc.date.issued1993-01-01
dc.description.abstractThe plating efficiency of standard mycological media such as brain heart infusion (BHI) agar is poor for Paracoccidioides brasiliensis. We prepared a water-extract of yeast phase cells of P. brasiliensis and examined it for growth-enhancing activity for the fungus. The water-extract, when added to BHI agar to a concentration of 5%, improved the plating efficiency of the medium for the fungus to some extent, but the degree of improvement was considerably varied among P. brasiliensis isolates. By contrast, when the water-extract was added in combination with horse serum (4%), the plating efficiency was highly improved (to 94-99%) for all the P. brasiliensis isolates employed. The growth-enhancing factor(s) in the water-extract was heat-stable and heating at 120-degrees-C for 15 min had little, if any, effect on growth-enhancing activity.en
dc.description.affiliationUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,FAC MED,DEPT PATHOL,BR-18618 BOTUCATU,BRAZIL
dc.description.affiliationUnespUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,FAC MED,DEPT PATHOL,BR-18618 BOTUCATU,BRAZIL
dc.format.extent201-205
dc.identifierhttp://dx.doi.org/10.1080/02681219380000251
dc.identifier.citationJournal of Medical and Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 31, n. 3, p. 201-205, 1993.
dc.identifier.doi10.1080/02681219380000251
dc.identifier.issn0268-1218
dc.identifier.urihttp://hdl.handle.net/11449/38154
dc.identifier.wosWOS:A1993LH01900003
dc.language.isoeng
dc.publisherBlackwell Science
dc.relation.ispartofJournal of Medical and Veterinary Mycology
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleAN IMPROVED CULTURE-MEDIUM FOR DETECTING LIVE YEAST PHASE CELLS OF PARACOCCIDIOIDES-BRASILIENSISen
dc.typeArtigo
dcterms.licensehttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
dcterms.rightsHolderBlackwell Science Ltd
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt
unesp.departmentPatologia - FMBpt

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