Two-year randomized clinical trial of different restorative techniques in non-carious cervical lesions and MMP activity in gingival crevicular fluid

dc.contributor.authorGonçalves, Rafael Simões [UNESP]
dc.contributor.authorScaffa, Polliana Mendes Candia
dc.contributor.authorShinohara, Mirela Sanae [UNESP]
dc.contributor.authorde Andrade Carvalho, Paulo Roberto Marão [UNESP]
dc.contributor.authorBuzalaf, Marília Afonso Rabelo
dc.contributor.authorFagundes, Ticiane Cestari [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionOregon Health and Science University
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2022-05-01T09:00:54Z
dc.date.available2022-05-01T09:00:54Z
dc.date.issued2022-02-01
dc.description.abstractObjectives: To evaluate different restorative techniques for non-carious cervical lesions (NCCLs) and the activity of matrix metalloproteinases (MMPs) in gingival crevicular fluid. Materials and methods: Two hundred restorations were performed in 50 patients using resin composite restorative system without (I) and with selective enamel conditioning (II) and resin-modified glass-ionomer cement without (III) and with EDTA pretreatment (IV). Gingival crevicular fluid samples were collected in 15 patients. Restorations were evaluated using USPHS criteria at baseline and after 2 years. Percentages of MMP activity were assessed by zymography as a surrogate outcome. Equality tests of two proportions, logistic regression analysis, survival analysis, ANOVA repeated measures, and Fisher tests were used. Results: No differences in clinical performance were found among groups. Group I had lower retention at 2 years than at baseline. Decreased alpha scores for marginal integrity and marginal discoloration were observed for all groups after 2 years. MMP-2 decreased after 1 year, and its activity increased back to the initial level after 2 years, mainly for groups I, II, and III. MMP-9 increased after 1 year, and it was reduced to the initial level after 2 years, mainly for group I. Conclusions: All restorative techniques performed similarly in NCCLs after 2 years with initial marginal defect alterations. MMP-2 reestablished its initial levels after 2 years, and MMP-9 had few alterations over time in crevicular fluid. Clinical relevance The different restorative techniques are equally successful in NCCLs after 2 years of clinical functioning and have similar effects on MMPs present in crevicular fluid.en
dc.description.affiliationAraçatuba School of Dentistry São Paulo State University, José Bonifácio St, 1193, SP
dc.description.affiliationOregon Health and Science University, SW Sam Jackson Park Rd, OR 97239
dc.description.affiliationBauru School of Dentistry University of São Paulo, Alameda Dr. Octávio Pinheiro Brisolla St., 9-75 - Jardim Brasil, SP
dc.description.affiliationUnespAraçatuba School of Dentistry São Paulo State University, José Bonifácio St, 1193, SP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2014/07086-0
dc.description.sponsorshipIdCNPq: 447616/2014-5
dc.format.extent1889-1902
dc.identifierhttp://dx.doi.org/10.1007/s00784-021-04166-2
dc.identifier.citationClinical Oral Investigations, v. 26, n. 2, p. 1889-1902, 2022.
dc.identifier.doi10.1007/s00784-021-04166-2
dc.identifier.issn1436-3771
dc.identifier.issn1432-6981
dc.identifier.scopus2-s2.0-85114681979
dc.identifier.urihttp://hdl.handle.net/11449/233519
dc.language.isoeng
dc.relation.ispartofClinical Oral Investigations
dc.sourceScopus
dc.subjectClinical trial
dc.subjectGingival crevicular fluid
dc.subjectGlass-ionomer cement
dc.subjectMatrix metalloproteinases
dc.subjectNon-carious cervical lesions
dc.subjectResin composite
dc.titleTwo-year randomized clinical trial of different restorative techniques in non-carious cervical lesions and MMP activity in gingival crevicular fluiden
dc.typeArtigo
unesp.author.orcid0000-0002-3418-0498[6]

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