PURIFICATION AND PROPERTIES OF SHIKIMATE DEHYDROGENASE FROM CUCUMBER (CUCUMIS-SATIVUS L)

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dc.contributor.authorLourenco, E. J.
dc.contributor.authorSilva, GML
dc.contributor.authorNeves, Valdir Augusto [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:20:58Z
dc.date.available2014-05-20T15:20:58Z
dc.date.issued1991-03-01
dc.description.abstractShikimate dehydrogenase (SDH, EC 1.1.1.25) extracted from cucumber pulp (Cucumis sativus L.) was purified 7-fold by precipitation with ammonium sulfate and elution from columns of Sephadex G-25, DEAE-cellulose, and hydroxyapatite. Two activity bands were detected on polyacrylamide gel electrophoresis at the last purification step. pH optimum was 8.7, and molecular weight of 45 000 was estimated on a Sephadex G-100 column. SDH was inhibited competitively by protocatechuic acid with a K(i) value of 2 x 10-4 M. K(m) values of 6 x 10-5 and 1 x 10-5 M were determined for shikimic acid and NADP+, respectively. The enzyme was completely inhibited by HgCl2 and p-(chloromercuri)benzoate (PCMB). NaCl and KCl showed partial protection against inhibition by PCMB. Heat inactivation between 50 and 55-degrees-C was biphasic, and the enzyme was completely inactivated after 10 min at 60-degrees-C. Incubation of SDH with either NADP+ or shikimic acid protected the enzyme against heat inactivation.en
dc.description.affiliationUNESP,FCF,DEPT FOOD & NUTR,RODOVIA ARARAQUARA JAU KM 1,CAIXA POSTAL 502,BR-14800 ARARAQUARA,BRAZIL
dc.description.affiliationUnespUNESP,FCF,DEPT FOOD & NUTR,RODOVIA ARARAQUARA JAU KM 1,CAIXA POSTAL 502,BR-14800 ARARAQUARA,BRAZIL
dc.format.extent458-462
dc.identifierhttp://dx.doi.org/10.1021/jf00003a006
dc.identifier.citationJournal of Agricultural and Food Chemistry. Washington: Amer Chemical Soc, v. 39, n. 3, p. 458-462, 1991.
dc.identifier.doi10.1021/jf00003a006
dc.identifier.issn0021-8561
dc.identifier.lattes4031319519910419
dc.identifier.urihttp://hdl.handle.net/11449/32155
dc.identifier.wosWOS:A1991FC49000006
dc.language.isoeng
dc.publisherAmer Chemical Soc
dc.relation.ispartofJournal of Agricultural and Food Chemistry
dc.relation.ispartofjcr3.412
dc.relation.ispartofsjr1,269
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titlePURIFICATION AND PROPERTIES OF SHIKIMATE DEHYDROGENASE FROM CUCUMBER (CUCUMIS-SATIVUS L)en
dc.typeArtigo
dcterms.licensehttp://pubs.acs.org/paragonplus/copyright/jpa_form_a.pdf
dcterms.rightsHolderAmer Chemical Soc
unesp.author.lattes4031319519910419
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentAlimentos e Nutrição - FCFpt

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Araraquara - FCF - Faculdade de Ciências Farmacêuticas