PURIFICATION AND PROPERTIES OF SHIKIMATE DEHYDROGENASE FROM CUCUMBER (CUCUMIS-SATIVUS L)
dc.contributor.author | Lourenco, E. J. | |
dc.contributor.author | Silva, GML | |
dc.contributor.author | Neves, Valdir Augusto [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-05-20T15:20:58Z | |
dc.date.available | 2014-05-20T15:20:58Z | |
dc.date.issued | 1991-03-01 | |
dc.description.abstract | Shikimate dehydrogenase (SDH, EC 1.1.1.25) extracted from cucumber pulp (Cucumis sativus L.) was purified 7-fold by precipitation with ammonium sulfate and elution from columns of Sephadex G-25, DEAE-cellulose, and hydroxyapatite. Two activity bands were detected on polyacrylamide gel electrophoresis at the last purification step. pH optimum was 8.7, and molecular weight of 45 000 was estimated on a Sephadex G-100 column. SDH was inhibited competitively by protocatechuic acid with a K(i) value of 2 x 10-4 M. K(m) values of 6 x 10-5 and 1 x 10-5 M were determined for shikimic acid and NADP+, respectively. The enzyme was completely inhibited by HgCl2 and p-(chloromercuri)benzoate (PCMB). NaCl and KCl showed partial protection against inhibition by PCMB. Heat inactivation between 50 and 55-degrees-C was biphasic, and the enzyme was completely inactivated after 10 min at 60-degrees-C. Incubation of SDH with either NADP+ or shikimic acid protected the enzyme against heat inactivation. | en |
dc.description.affiliation | UNESP,FCF,DEPT FOOD & NUTR,RODOVIA ARARAQUARA JAU KM 1,CAIXA POSTAL 502,BR-14800 ARARAQUARA,BRAZIL | |
dc.description.affiliationUnesp | UNESP,FCF,DEPT FOOD & NUTR,RODOVIA ARARAQUARA JAU KM 1,CAIXA POSTAL 502,BR-14800 ARARAQUARA,BRAZIL | |
dc.format.extent | 458-462 | |
dc.identifier | http://dx.doi.org/10.1021/jf00003a006 | |
dc.identifier.citation | Journal of Agricultural and Food Chemistry. Washington: Amer Chemical Soc, v. 39, n. 3, p. 458-462, 1991. | |
dc.identifier.doi | 10.1021/jf00003a006 | |
dc.identifier.issn | 0021-8561 | |
dc.identifier.lattes | 4031319519910419 | |
dc.identifier.uri | http://hdl.handle.net/11449/32155 | |
dc.identifier.wos | WOS:A1991FC49000006 | |
dc.language.iso | eng | |
dc.publisher | Amer Chemical Soc | |
dc.relation.ispartof | Journal of Agricultural and Food Chemistry | |
dc.relation.ispartofjcr | 3.412 | |
dc.relation.ispartofsjr | 1,269 | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Web of Science | |
dc.title | PURIFICATION AND PROPERTIES OF SHIKIMATE DEHYDROGENASE FROM CUCUMBER (CUCUMIS-SATIVUS L) | en |
dc.type | Artigo | |
dcterms.license | http://pubs.acs.org/paragonplus/copyright/jpa_form_a.pdf | |
dcterms.rightsHolder | Amer Chemical Soc | |
unesp.author.lattes | 4031319519910419 | |
unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquara | pt |
unesp.department | Alimentos e Nutrição - FCF | pt |
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