Morphological alterations induced by boric acid and fipronil in the midgut of worker honeybee (Apis mellifera L.) larvae

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dc.contributor.authorCruz, Aline da Silva [UNESP]
dc.contributor.authorda Silva-Zacarin, Elaine C. M.
dc.contributor.authorBueno, Odair C. [UNESP]
dc.contributor.authorMalaspina, Osmar [UNESP]
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:55:20Z
dc.date.available2014-05-20T13:55:20Z
dc.date.issued2010-04-01
dc.description.abstractMorphological alterations, by means of histological and ultrastructural analysis, have been used to determine the effects of boric acid and fipronil on midgut tissues of honeybee worker, Apis mellifera L. larvae. In order to observe possible morphological alterations in the midgut, two groups of bioassays were performed. In the first one, the larvae were chronically treated with different concentrations of boric acid added to the food (1.0, 2.5 and 7.5 mg/g). In the second group, the larvae were fed with diets containing different concentrations of fipronil (0.1 and 1 mu g/g) and compared with control groups without these chemical compounds. In the first bioassay, the larvae were collected on day 3 and in the second bioassay on day 4, when the mortality rate obtained in the toxicological bioassay was not very high. The larval midguts were removed and processed for morphological analyses using a light and transmission electron microscopy. We observed cytoplasmic vacuolizations, with the absence of autophagic vacuoles, and chromatinic compacting in most of the cells in the groups treated with pesticides. The morphological alterations were far greater in the larvae treated with boric acid than in the larvae treated with fipronil. Our data suggest that the midgut cell death observed was in response to boric acid and fipronil action. This study significantly improves the understanding of the toxicological effect of these insecticides from the ecotoxicological perspective.en
dc.description.affiliationUniversidade Federal de São Carlos (UFSCar), Funct & Struct Biol Lab, Sorocaba, SP, Brazil
dc.description.affiliationUNESP, CEIS Social Insects Studies Ctr, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUNESP, CEIS Social Insects Studies Ctr, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipDepartment of Biology at UNESP- Rio Claro
dc.format.extent165-176
dc.identifierhttp://dx.doi.org/10.1007/s10565-009-9126-x
dc.identifier.citationCell Biology and Toxicology. Dordrecht: Springer, v. 26, n. 2, p. 165-176, 2010.
dc.identifier.doi10.1007/s10565-009-9126-x
dc.identifier.issn0742-2091
dc.identifier.lattes7538556085505819
dc.identifier.orcid0000-0002-1650-257X
dc.identifier.urihttp://hdl.handle.net/11449/19805
dc.identifier.wosWOS:000275090000008
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofCell Biology and Toxicology
dc.relation.ispartofjcr3.390
dc.relation.ispartofsjr0,924
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectUltrastructureen
dc.subjectHistologyen
dc.subjectBoric aciden
dc.subjectFipronilen
dc.subjectMidguten
dc.subjectHoneybee larvaeen
dc.titleMorphological alterations induced by boric acid and fipronil in the midgut of worker honeybee (Apis mellifera L.) larvaeen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes7538556085505819[4]
unesp.author.orcid0000-0002-1650-257X[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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Artigos - Biologia - IBRC