Proteome profiling reveals insights into secondary metabolism in Maytenus ilicifolia (Celastraceae) cell cultures producing quinonemethide triterpenes

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dc.contributor.authorPaz, Tiago Antunes [UNESP]
dc.contributor.authordos Santos, Vânia A. F. F. M. [UNESP]
dc.contributor.authorInácio, Marielle Cascaes [UNESP]
dc.contributor.authorDias, Nathalia Batista [UNESP]
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.contributor.authorPereira, Ana Maria Soares
dc.contributor.authorFurlan, Maysa [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de Ribeirão Preto
dc.date.accessioned2018-12-11T17:32:44Z
dc.date.available2018-12-11T17:32:44Z
dc.date.issued2017-08-01
dc.description.abstractPlants produce a wide variety of secondary metabolites (SMs) of pharmacological interest that, due to their high structural complexity, are difficult to obtain from chemical synthesis. Knowledge of the enzymatic process involved in the biosynthesis of these SMs may contribute to the development of new strategies for obtaining them from plant tissues. Maytenus ilicifolia contains three main classes of bioactive compounds: sesquiterpene pyridine alkaloids, flavonoids and, primarily, quinonemethide triterpenes, which are well known for their high and broad antitumor activity. Thus, to determine the enzymatic composition in the secondary metabolism of M. ilicifolia, we carried out proteomic profiling of M. ilicifolia cell cultures with and without increased concentrations of maytenin and 22β-hydroxy-maytenin by elicitation with methyl jasmonate for 48 h. For this purpose, the analysis of soluble proteins was performed via LCMS/MS experiments using a shotgun strategy and compared to specific databases, resulting in 1319 identifications in the two types of cells. Many enzymes involved in secondary metabolism were detected, including cytochrome P450-dependent monooxygenases, which potentially catalyze the final steps in the quinonemethide triterpene biosynthesis. In conclusion, we report for the first time a proteomic study of a cell culture system that is an effective elicitor of quinonemethide triterpene production.en
dc.description.affiliationInstituto de Química Univ. Estadual Paulista - UNESP, Rua Prof. Francisco Degni, 55, Quitandinha
dc.description.affiliationInstituto de Biociências Univ. Estadual Paulista - UNESP
dc.description.affiliationDepartamento de Biotecnologia Vegetal Universidade de Ribeirão Preto
dc.description.affiliationUnespInstituto de Química Univ. Estadual Paulista - UNESP, Rua Prof. Francisco Degni, 55, Quitandinha
dc.description.affiliationUnespInstituto de Biociências Univ. Estadual Paulista - UNESP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2011/01544-9
dc.description.sponsorshipIdFAPESP: 2014/19362-2
dc.format.extent405-416
dc.identifierhttp://dx.doi.org/10.1007/s11240-017-1236-1
dc.identifier.citationPlant Cell, Tissue and Organ Culture, v. 130, n. 2, p. 405-416, 2017.
dc.identifier.doi10.1007/s11240-017-1236-1
dc.identifier.file2-s2.0-85020138529.pdf
dc.identifier.issn1573-5044
dc.identifier.issn0167-6857
dc.identifier.lattes2901888624506535
dc.identifier.lattes1308042794786872
dc.identifier.scopus2-s2.0-85020138529
dc.identifier.urihttp://hdl.handle.net/11449/178927
dc.language.isoeng
dc.relation.ispartofPlant Cell, Tissue and Organ Culture
dc.relation.ispartofsjr0,855
dc.relation.ispartofsjr0,855
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCell cultures
dc.subjectElicitation
dc.subjectMethyl jasmonate
dc.subjectProteomics
dc.subjectQuinonemethide triterpenes
dc.titleProteome profiling reveals insights into secondary metabolism in Maytenus ilicifolia (Celastraceae) cell cultures producing quinonemethide triterpenesen
dc.typeArtigo
unesp.advisor.lattes2901888624506535
unesp.author.lattes1308042794786872
unesp.author.orcid0000-0002-8434-5238[7]

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Artigos - Biologia - IBRC
Artigos - Química Orgânica - IQ