Effect of Silver Nanoparticles in a Hydroxyapatite Coating applied by Atmospheric Plasma Spray

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dc.contributor.authorOrozco Carmona, V.
dc.contributor.authorMartinez Perez, C.
dc.contributor.authorLima, Renata de
dc.contributor.authorFernandes Fraceto, Leonardo [UNESP]
dc.contributor.authorRomero Garcia, J.
dc.contributor.authorLedezma Perez, A.
dc.contributor.authorMarke, Swen
dc.contributor.authorRodriguez Gonzalez, C.
dc.contributor.authorHurtado Macias, Abel
dc.contributor.authorMartinez-Villafane, A.
dc.contributor.institutionCtr Invest Mat Avanzados CIMAV
dc.contributor.institutionUniv Autonoma Ciudad Juarez
dc.contributor.institutionUniv Sorocaba
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionCtr Invest Quim Aplicada
dc.contributor.institutionIfU Diagnost Sytems GmbH
dc.date.accessioned2015-03-18T15:52:31Z
dc.date.available2015-03-18T15:52:31Z
dc.date.issued2014-12-01
dc.description.abstractIn this study, 0.5, 1, 3, and 5 % nano-silver containing hydroxyapatite coatings (nAg-HA) on a Ti6Al4V substrate were developed by atmospheric plasma spray (APS), and their antibacterial efficiency was evaluated in the following bacterial strains: Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. The optimal operating parameters for the coatings application were determined by online diagnostic of thermal HVOF- and plasma spraying processes. Scanning electron microscopy (SEM) showed that both micro and nano-silver (Ag) particles were distributed on the coating surface. The antibacterial efficiency was studied according to the JIS Z2801:2000 standard Antimicrobial products-Test for antimicrobial activity and efficacy. The results show that the antibacterial efficiency of a 1 % nAg-HA coating against Escherichia coli, Pseudomonas aeruginosa, and Pseudomonas aeruginosa strains was above 99 % antibacterial rate. Silver ion release tests show that the coatings did not deliver the silver ions in the phosphate buffered saline solution, which generate good cytotoxic properties. The antibacterial mechanisms observed in the coatings are based in the combination of the two following theories proposed by Cao: 1) Disruption of transmembrane proton electrochemical gradient, and 2) bacterial charging process. The electrochemical test in SBF solution at 25 and 37 degrees C showed an i(corr) higher than 25.00E-6 A/cm(2), which justified the good antibacterial properties. The nAg-HA coatings developed presented excellent bond strength (35 MPa in average), nano-mechanical properties, and the failure mode was identified as adhesion-cohesion. This study opens perspectives for the development of in-vivo tests of the nAg-HA coatings applied by APS; research in an alternative method to enhance the nano-particles dispersion into the HA matrix; and finally, the study suggests further research to determine which of the two mechanisms proposed by Cao presents the major effect in the antibacterial rate.en
dc.description.affiliationCtr Invest Mat Avanzados CIMAV, Chihuahua 31109, Mexico
dc.description.affiliationUniv Autonoma Ciudad Juarez, Juarez City 32310, Mexico
dc.description.affiliationUniv Sorocaba, Dept Biotecnol, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Carlos, UFSCar, Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Engn Ambiental, Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Bioquim, Campinas, SP, Brazil
dc.description.affiliationCtr Invest Quim Aplicada, Saltillo 25250, Coahuila, Mexico
dc.description.affiliationIfU Diagnost Sytems GmbH, D-09244 Lichtenau, Germany
dc.description.affiliationUnespUniv Estadual Paulista, Dept Engn Ambiental, Sao Paulo, Brazil
dc.description.sponsorshipConacyt
dc.description.sponsorshipEuropean Union Seventh Framework Programme FP7
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação para o Desenvolvimento da UNESP (FUNDUNESP)
dc.description.sponsorshipIdConacytC0006-2009-01/127139
dc.description.sponsorshipIdEuropean Union Seventh Framework Programme FP7NMP4-CP-2011-263942
dc.format.extent7471-7494
dc.identifierhttp://connection.ebscohost.com/c/articles/99328059/effect-silver-nanoparticles-hydroxyapatite-coating-applied-by-atmospheric-plasma-spray
dc.identifier.citationInternational Journal Of Electrochemical Science. Belgrade: Esg, v. 9, n. 12, p. 7471-7494, 2014.
dc.identifier.issn1452-3981
dc.identifier.urihttp://hdl.handle.net/11449/116170
dc.identifier.wosWOS:000345261900060
dc.language.isoeng
dc.publisherEsg
dc.relation.ispartofInternational Journal Of Electrochemical Science
dc.relation.ispartofjcr1.369
dc.relation.ispartofsjr0,366
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectSilver nano particlesen
dc.subjectHydroxyapatite coatingen
dc.subjectAtmospheric Plasma Sprayen
dc.subjectAntibacterial Testen
dc.subjectCytotoxic Testen
dc.subjectElectrochemical Testen
dc.titleEffect of Silver Nanoparticles in a Hydroxyapatite Coating applied by Atmospheric Plasma Sprayen
dc.typeArtigo
dcterms.rightsHolderEsg
unesp.author.orcid0000-0001-8712-1144[3]
unesp.author.orcid0000-0002-2827-2038[4]
unesp.author.orcid0000-0002-0676-279X[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Ciência e Tecnologia, Sorocabapt

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