Live and ultrasound-inactivated Lacticaseibacillus casei modulate the intestinal microbiota and improve biochemical and cardiovascular parameters in male rats fed a high-fat diet

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dc.contributor.authorBrandao, Larissa Ramalho
dc.contributor.authorBrito Alves, Jose Luiz de
dc.contributor.authorCosta, Whyara Karoline Almeida da
dc.contributor.authorFerreira, Georgianna de Araujo Henriques
dc.contributor.authorOliveira, Matthaws Pereira de
dc.contributor.authorGomes da Cruz, Adriano
dc.contributor.authorBraga, Valdir de Andrade
dc.contributor.authorAquino, Jailane de Souza
dc.contributor.authorVidal, Hubert
dc.contributor.authorNoronha, Melline Fontes
dc.contributor.authorCabral, Lucelia [UNESP]
dc.contributor.authorPimentel, Tatiana Colombo
dc.contributor.authorMagnani, Marciane
dc.contributor.institutionUniv Fed Paraiba
dc.contributor.institutionFed Inst Sci & Technol Rio De Janeiro
dc.contributor.institutionUniv Claude Bernard Lyon 1
dc.contributor.institutionUniv Illinois
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFed Inst Parana
dc.date.accessioned2021-06-25T15:05:33Z
dc.date.available2021-06-25T15:05:33Z
dc.date.issued2021-05-08
dc.description.abstractThis study aimed to evaluate the effects of ingestion of live (9 log CFU mL(-1)) and ultrasound-inactivated (paraprobiotic, 20 kHz, 40 min) Lacticaseibacillus casei 01 cells for 28 days on healthy parameters (biochemical and cardiovascular) and intestinal microbiota (amplicon sequencing of 16S ribosomal RNA) of rats fed a high-fat diet. Twenty-four male Wistar rats were divided into four groups of six animals: CTL (standard diet), HFD (high-fat diet), HFD-LC (high-fat diet and live L. casei), and HFD-ILC (high-fat diet and inactivated L. casei). The administration of live and ultrasound-inactivated L. casei prevented the increase (p < 0.05) in cholesterol levels (total and LDL) and controlled the insulin resistance in rats fed a high-fat diet. Furthermore, it promoted a modulation of the intestinal microbial composition by increasing (p < 0.05) beneficial bacteria (Lachnospiraceae and Ruminoccocaceae) and decreasing (p < 0.05) harmful bacteria (Clostridiaceae, Enterobacteriaceae, and Helicobacteriacea), attenuating the effects promoted by the HFD ingestion. Only live cells could increase (p < 0.05) the HDL-cholesterol, while only inactivated cells caused attenuation (p < 0.05) of the blood pressure. Results show beneficial effects of live and inactivated L. casei 01 and indicate that ultrasound inactivation produces a paraprobiotic with similar or improved health properties compared to live cells.en
dc.description.affiliationUniv Fed Paraiba, Dept Food Engn, Technol, Joao Pessoa, Paraiba, Brazil
dc.description.affiliationUniv Fed Paraiba, Hlth Sci Ctr, Dept Nutr, Joao Pessoa, Paraiba, Brazil
dc.description.affiliationFed Inst Sci & Technol Rio De Janeiro, Dept Food, Rio De Janeiro, RJ, Brazil
dc.description.affiliationUniv Fed Paraiba, Dept Biotechnol, Biotechnol Ctr, Joao Pessoa, Paraiba, Brazil
dc.description.affiliationUniv Claude Bernard Lyon 1, Univ Lyon, CarMeN Cardio Metab Diabet & Nutr Lab, INSERM,INRAE,INSA Lyon, Oullins, France
dc.description.affiliationUniv Illinois, Res Resource Ctr, Res Informat Core, Chicago, IL USA
dc.description.affiliationSao Paulo State Univ, Dept Gen & Appl Biol, Inst Biosci, Rio Claro, SP, Brazil
dc.description.affiliationFed Inst Parana, Campus Paranavai, Paranavai, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Dept Gen & Appl Biol, Inst Biosci, Rio Claro, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundacao de Apoio a Pesquisa do Estado da Paraiba (FAPESQ)
dc.description.sponsorshipIdCNPq: 303437/2017-0
dc.description.sponsorshipIdCNPq: 405644/2018-3
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdCAPES: UFPB PrInt 88881.311776/2018-01
dc.description.sponsorshipIdFundacao de Apoio a Pesquisa do Estado da Paraiba (FAPESQ): 007/2019 FAPESQ-PB-MCT/CNPq
dc.format.extent14
dc.identifierhttp://dx.doi.org/10.1039/d1fo01064f
dc.identifier.citationFood & Function. Cambridge: Royal Soc Chemistry, 14 p., 2021.
dc.identifier.doi10.1039/d1fo01064f
dc.identifier.issn2042-6496
dc.identifier.urihttp://hdl.handle.net/11449/210346
dc.identifier.wosWOS:000651904500001
dc.language.isoeng
dc.publisherRoyal Soc Chemistry
dc.relation.ispartofFood & Function
dc.sourceWeb of Science
dc.titleLive and ultrasound-inactivated Lacticaseibacillus casei modulate the intestinal microbiota and improve biochemical and cardiovascular parameters in male rats fed a high-fat dieten
dc.typeArtigo
dcterms.rightsHolderRoyal Soc Chemistry

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