Effects of physiological levels of daidzein on cell proliferation of tumoral and non-tumoral breast cells lines

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dc.contributor.authorTsuboy, Marcela Stefanini Ferreira [UNESP]
dc.contributor.authorMarcarini, Juliana Cristina [UNESP]
dc.contributor.authorSouza, Alecsandra Oliveira de
dc.contributor.authorPaula, Natalia Aparecida de
dc.contributor.authorLuiz, Rodrigo Cabral
dc.contributor.authorDorta, Daniel Junqueira
dc.contributor.authorMantovani, Mário Sérgio
dc.contributor.authorRibeiro, Lucia Regina [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.date.accessioned2016-07-07T12:38:18Z
dc.date.available2016-07-07T12:38:18Z
dc.date.issued2014
dc.description.abstractIntroduction: Soy isoflavones are associated with the lower breast cancer risk found in Asiatic populations. They can interfere in various stages of the carcinogenesis process, preventing the development of this pathology and, for this reason, its consumption became popular. Instead of the beneficial effects found in literature, most chemopreventive in vitro studies use non-physiological levels of daidzein (> 30 µM). In our study, the effects of maximal physiological levels of daidzein was investigated in two human breast cell lines: HB4a (non-tumoral) and MCF-7 (tumoral). Experiments to verify cytotoxicity, induction of cell death by apoptosis and, additionally, cell cycle arrest were performed. Materials and methods: Cytotoxic effect of daidzein (0.1 - 100 µM) was assessed using resazurinbased assay. Flow cytometry tests were performed to investigate apoptosis induction (annexin VFITC/PI) and cell cycle arrest (propidium iodide staining of DNA content) by daidzein at 10 and 25 µM. Changes in expression of apoptosis related genes after treatment of cells with daidzein (10 and 25 µM) were investigated using quantitative real time PCR. Results: Daidzein ranging from 0.1 to 100 µM (for 24 and 48h) were not cytotoxic to both cell lines. Concentrations of 10 and 25 µM did not induce apoptosis in these cells, instead of statistical significant changes in gene expression of some of the target genes (CASP-3, CASP-7, BAX, BCL-xL, COX-2). Both concentrations of daidzein arrested cell cycle in G0/G1 phase only in MCF- 7. Conclusion: Daidzein at maximal achievable physiologic serum levels selectively arrested cell cycle only in the tumoral MCF-7, and did not induce cell death by apoptosis, one of the major effects described in literature. We hope that our study helps in understanding the chemopreventive effects of low levels of soy isoflavones in breast carcinogenesis.en
dc.description.affiliationUniversidade de São Paulo, Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto
dc.description.affiliationUniversidade de São Paulo, Departamento de Clínica Médica, Faculdade de Medicina de Ribeirão Preto
dc.description.affiliationUniversidade Estadual de Londrina, Departamento de Patologia
dc.description.affiliationUniversidade Estadual de Londrina, Departamento de Biologia
dc.description.affiliationUnespUniversidade Estadual Paulista, Instituto de Biociências de Rio Claro
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdCNPq: 471938/2008-4
dc.format.extent1-9
dc.identifierhttp://www.oapublishinglondon.com/article/1296
dc.identifier.citationOA Cancer, v. 2, n. 1, p. 1-9, 2014.
dc.identifier.fileISSN2053-3918-2014-02-01-01-09.pdf
dc.identifier.issn2053-3918
dc.identifier.lattes7787314478210146
dc.identifier.lattes7965342877016058
dc.identifier.lattes7150764285887192
dc.identifier.lattes1779939619083787
dc.identifier.lattes6630970920236636
dc.identifier.lattes6339105541634538
dc.identifier.lattes9570878174185086
dc.identifier.urihttp://hdl.handle.net/11449/141434
dc.language.isoeng
dc.relation.ispartofOA Cancer
dc.rights.accessRightsAcesso aberto
dc.sourceCurrículo Lattes
dc.titleEffects of physiological levels of daidzein on cell proliferation of tumoral and non-tumoral breast cells linesen
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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