The influence of polysaccharide coating on the physicochemical parameters and cytotoxicity of silica nanoparticles for hydrophilic biomolecules delivery

Página do item simplificado
dc.contributor.authorAndreani, Tatiana
dc.contributor.authorFangueiro, Joana F.
dc.contributor.authorSeverino, Patrícia
dc.contributor.authorde Souza, Ana Luiza R. [UNESP]
dc.contributor.authorMartins-Gomes, Carlos
dc.contributor.authorFernandes, Paula M. V.
dc.contributor.authorCalpena, Ana C.
dc.contributor.authorGremião, Maria P. [UNESP]
dc.contributor.authorSouto, Eliana B.
dc.contributor.authorSilva, Amélia M.
dc.contributor.institutionUniversity of Trás-os-Montes e Alto Douro
dc.contributor.institutionPorto University
dc.contributor.institutionUniversity of Tiradentes
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Barcelona
dc.contributor.institutionUniversity of Coimbra (FFUC)
dc.contributor.institutionUniversity of Minho
dc.date.accessioned2019-10-06T15:51:58Z
dc.date.available2019-10-06T15:51:58Z
dc.date.issued2019-07-01
dc.description.abstractThe present work reports the effect of polysaccharides (chitosan and sodium alginate) on silica nanoparticles (SiNP) for hydrophilic molecules delivery taking insulin as model drug. The influence of tetraethyl orthosilicate (TEOS) and homogenization speed on SiNP properties was assessed by a 22 factorial design achieving as optimal parameters: 0.43 mol/L of TEOS and homogenization speed of 5000 rpm. SiNP mean particle size (Z-Ave) was of 256.6 nm and polydispersity index (PI) of 0.218. SiNP coated with chitosan (SiNP-CH) or sodium alginate (SiNP-SA) increased insulin association efficacy; reaching 84.6% (SiNP-SA) and 90.8% (SiNP-CH). However, coated SiNP released 50%–60% of the peptide during the first 45 min at acidic environment, while uncoated SiNP only released ∼30%. Similar results were obtained at pH 6.8. The low Akaike’s (AIC) values indicated that drug release followed Peppas model for SiNP-SA and second order for uncoated SiNP and SiNP-CH (pH 2.0). At pH 6.8, the best fitting was Boltzmann for Ins-SiNP. However, SiNP-CH and SiNP-SA showed a first-order behavior. Cytotoxicity of nanoparticles, assessed in Caco-2 and HepG2 cells, showed that 100 to 500 µg/mL SiNP-CH and SiNP-SA slightly decreased cell viability, comparing with SiNP. In conclusion, coating SiNP with selected polysaccharides influenced the nanoparticles physicochemical properties, the insulin release, and the effect of these nanoparticles on cell viability.en
dc.description.affiliationCITAB-Centre for Research and Technology of Agro-Environmental and Biological Sciences University of Trás-os-Montes e Alto Douro, Quinta de Prados
dc.description.affiliationDepartment of Biology and Environment University of Trás-os-Montes e Alto Douro
dc.description.affiliationCIQUP-Research Center in Chemistry Department of Chemistry and Biochemistry Faculty of Sciences Porto University, Rua do Campo Alegre, s/n
dc.description.affiliationInstitute of Technology and Research University of Tiradentes, Avenida Murilo Dantas, Farolândia
dc.description.affiliationFaculty of Pharmaceutical Sciences Universidade Estadual Paulista UNESP, Rodovia Araraquara-Jau, Km. 01
dc.description.affiliationBiopharmacy and Pharmacokinetic Unit Pharmacy and Pharmaceutical Technology Department School of Pharmacy University of Barcelona, Av. Joan XXIII, s/n
dc.description.affiliationDepartment of Pharmaceutical Technology Faculty of Pharmacy University of Coimbra (FFUC), Pólo das Ciências da Saúde, Azinhaga de Santa Comba
dc.description.affiliationCEB-Centre of Biological Engineering University of Minho Campus de Gualtar
dc.description.affiliationUnespFaculty of Pharmaceutical Sciences Universidade Estadual Paulista UNESP, Rodovia Araraquara-Jau, Km. 01
dc.description.sponsorshipFundação para a Ciência e a Tecnologia
dc.description.sponsorshipIdFundação para a Ciência e a Tecnologia: NET/0004/2015-PAIRED
dc.description.sponsorshipIdFundação para a Ciência e a Tecnologia: SFRH/BD/111274/2015
dc.description.sponsorshipIdFundação para a Ciência e a Tecnologia: SFRH/BD/60640/2009
dc.description.sponsorshipIdFundação para a Ciência e a Tecnologia: SFRH/BD/80335/2011
dc.description.sponsorshipIdFundação para a Ciência e a Tecnologia: UID/AGR/04033/2019
dc.identifierhttp://dx.doi.org/10.3390/nano9081081
dc.identifier.citationNanomaterials, v. 9, n. 8, 2019.
dc.identifier.doi10.3390/nano9081081
dc.identifier.issn2079-4991
dc.identifier.scopus2-s2.0-85070412332
dc.identifier.urihttp://hdl.handle.net/11449/187941
dc.language.isoeng
dc.relation.ispartofNanomaterials
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCell toxicity
dc.subjectFactorial design
dc.subjectHydrophilic biomolecules
dc.subjectInsulin
dc.subjectKinetic studies
dc.subjectPolysaccharides
dc.subjectSilica nanoparticles
dc.titleThe influence of polysaccharide coating on the physicochemical parameters and cytotoxicity of silica nanoparticles for hydrophilic biomolecules deliveryen
dc.typeArtigo

Arquivos

Coleções

Artigos