In vitro and in silico cytotoxicity of hinokinin-loaded PLGA microparticle systems against tumoral SiHa cells

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dc.contributor.authorde Lima, Regiane G. [UNESP]
dc.contributor.authorLisoni, Flavia C. R. [UNESP]
dc.contributor.authorPicão, Thais B. [UNESP]
dc.contributor.authordos Santos, Fransérgio F.
dc.contributor.authorOrenha, Renato P.
dc.contributor.authorBorges, Alexandre
dc.contributor.authorMolina, Eduardo F.
dc.contributor.authorParreira, Renato L. T.
dc.contributor.authore Silva, Márcio L. A.
dc.contributor.authorSantos, Mario F. C.
dc.contributor.authorde Laurentiz, Rosangela da S. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de Franca
dc.contributor.institutionCentro Universitário
dc.date.accessioned2022-04-29T08:36:12Z
dc.date.available2022-04-29T08:36:12Z
dc.date.issued2021-01-01
dc.description.abstractThis work aimed to synthesize poly (D, L-lactic-co-glycolic acid) (PLGA) microparticles containing hinokinin (HNK) and to evaluate their cytotoxic activity against tumoral SiHa cells and non-tumoral HaCaT cells. Hinokinin was incorporated into PLGA (PLGA-HNK) with an encapsulation efficiency of 84.18 ± 2.32%. PLGA and PLGA-HNK were characterized by SEM microscopy and showed spherical morphology with an average size of ∼3.33. Encapsulation efficiency was determined by a calibration curve using UV-vis spectroscopy. PLGA-HNK more active inhibiting proliferation of SiHa cells (IC50 = 14.68 µM) than free HNK (IC50 = 225.5 µM). In relation to HaCaT cells, PLGA-HNK showed no significant difference compared to the negative control. These results led to an increase in HNK bioavailability and thereby, biological activity. In silico prediction analysis suggests that HNK is cytotoxic against SiHa cells with E6 and MDM2 inhibition as possible main mechanism of action.en
dc.description.affiliationDepartamento de Física e Química Faculdade de Engenharia de Ilha Solteira Universidade Estadual Paulista
dc.description.affiliationDepartamento de Biologia e Zootecnia Faculdade de Engenharia de Ilha Solteira Universidade Estadual Paulista
dc.description.affiliationNúcleo de Pesquisa em Ciências Exatas e Tecnológicas Universidade de Franca
dc.description.affiliationCentro Universitário
dc.description.affiliationUnespDepartamento de Física e Química Faculdade de Engenharia de Ilha Solteira Universidade Estadual Paulista
dc.description.affiliationUnespDepartamento de Biologia e Zootecnia Faculdade de Engenharia de Ilha Solteira Universidade Estadual Paulista
dc.identifierhttp://dx.doi.org/10.1080/14786419.2021.2000409
dc.identifier.citationNatural Product Research.
dc.identifier.doi10.1080/14786419.2021.2000409
dc.identifier.issn1478-6427
dc.identifier.issn1478-6419
dc.identifier.scopus2-s2.0-85118588907
dc.identifier.urihttp://hdl.handle.net/11449/229841
dc.language.isoeng
dc.relation.ispartofNatural Product Research
dc.sourceScopus
dc.subjectinhibition proliferation assay
dc.subjectmolecular docking
dc.subjectNatural products
dc.subjectPiper cubeba
dc.titleIn vitro and in silico cytotoxicity of hinokinin-loaded PLGA microparticle systems against tumoral SiHa cellsen
dc.typeArtigo

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Artigos - Física e Química - FEIS
Artigos - Biologia e Zootecnia - FEIS