Gels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitro

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dc.contributor.authorReis, Fabiana Navas
dc.contributor.authorFrancese, Monique Malta
dc.contributor.authorSilva, Natara Dias Gomes da
dc.contributor.authorPelá, Vinicius Taioqui
dc.contributor.authorCâmara, João Victor Frazão
dc.contributor.authorTrevizol, Juliana Sanches
dc.contributor.authorPieretti, Joana Claudio
dc.contributor.authorSeabra, Amedea Barozzi
dc.contributor.authorPessan, Juliano Pelim [UNESP]
dc.contributor.authorHonório, Heitor Marques
dc.contributor.authorCrusca, Edson [UNESP]
dc.contributor.authorMarchetto, Reinaldo [UNESP]
dc.contributor.authorBuzalaf, Marília Afonso Rabelo
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionFederal University of ABC
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:27:36Z
dc.date.available2023-07-29T13:27:36Z
dc.date.issued2023-01-01
dc.description.abstractThe effect of gels containing a statherin-derived peptide (Stn) on the protection against enamel and dentin erosive tooth wear (ETW) in vitro was evaluated. Bovine enamel and dentin specimens were divided into 2 groups (n = 15 and 18/group for enamel and dentin, respectively) that were treated with Chitosan or Carboxymethylcellulose (CMC) gels containing Stn15pSpS at 1.88 × 10-5 M or 3.76 × 10-5 M. Chitosan or CMC gels without active ingredients served as negative controls, while chitosan gel containing 1.23% F (as NaF) and acidulated phosphate fluoride gel (1.23% F) served as positive controls. The gels were applied on the specimens for 4 min. Stimulated saliva was collected from 3 donors and used to form a 2-h acquired pellicle on the specimens. Then, the specimens were submitted to an erosive pH cycling protocol 4 times/day for 7 days (0.01 M HCl pH 2.0/45 s, artificial saliva/2 h, and artificial saliva overnight). The gels were applied again during pH cycling, 2 times/day for 4 min after the first and last erosive challenges. Enamel and dentin loss (μm) were assessed by contact profilometry. Scanning electron microscopy (SEM) was analyzed using a cold field emission. Data were analyzed by two-way ANOVA (for chitosan and CMC gels, separately) and Tukey's multiple comparison test. SEM images showed changes to enamel topography after application oft the gels containing Stn or F. Regarding CMC-based gels, for enamel, none of the treatments significantly reduced ETW in comparison with placebo; for dentin, however, gels containing Stn, regardless the concentration, significantly reduced the ETW. Moreover, Chitosan-based gels, regardless the Stn concentration, were able to protect enamel and dentin against ETW. Gels containing Stn might be a new approach to protect against ETW.en
dc.description.affiliationDepartment of Biological Sciences Bauru School of Dentistry University of São Paulo
dc.description.affiliationCenter for Natural and Human Sciences Federal University of ABC
dc.description.affiliationDepartment of Pediatric Dentistry and Public Health Araçatuba Dental School São Paulo State University UNESP, Araçatuba
dc.description.affiliationChemistry Institute Paulista State University
dc.description.affiliationUnespDepartment of Pediatric Dentistry and Public Health Araçatuba Dental School São Paulo State University UNESP, Araçatuba
dc.description.affiliationUnespChemistry Institute Paulista State University
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2019/26070–1
dc.identifierhttp://dx.doi.org/10.1016/j.jmbbm.2022.105549
dc.identifier.citationJournal of the Mechanical Behavior of Biomedical Materials, v. 137.
dc.identifier.doi10.1016/j.jmbbm.2022.105549
dc.identifier.issn1878-0180
dc.identifier.issn1751-6161
dc.identifier.scopus2-s2.0-85141529742
dc.identifier.urihttp://hdl.handle.net/11449/247849
dc.language.isoeng
dc.relation.ispartofJournal of the Mechanical Behavior of Biomedical Materials
dc.sourceScopus
dc.subjectDental pellicle
dc.subjectHuman
dc.subjectStatherin protein
dc.subjectTooth erosion
dc.titleGels containing statherin-derived peptide protect against enamel and dentin erosive tooth wear in vitroen
dc.typeArtigo
unesp.author.orcid0000-0002-6692-2273[3]
unesp.author.orcid0000-0002-9687-4401[5]
unesp.author.orcid0000-0002-7519-049X[11]
unesp.author.orcid0000-0002-8757-321X[12]
unesp.author.orcid0000-0002-5985-3951[13]

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Artigos - Odontologia Infantil e Social - FOA