Corneal angiogenesis based on different protocols of alkaline cauterization in murine models

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dc.contributor.authorClaros-Chacaltana, Flor Diana Yokoay [UNESP]
dc.contributor.authorKobashigawa, Karina Kamachi [UNESP]
dc.contributor.authorPadua, Ivan Ricardo Martinez [UNESP]
dc.contributor.authorValdetaro, Gisele Pereira [UNESP]
dc.contributor.authorAldrovani, Marcela [UNESP]
dc.contributor.authorLaus, José Luiz [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:34:04Z
dc.date.available2018-12-11T17:34:04Z
dc.date.issued2017-01-01
dc.description.abstractPurpose: To establish and compare protocols of alkaline cauterization for inducing corneal angiogenesis in murine models. Methods: Twenty-four adult Wistar rats were distributed into four groups (G1, G2, G3, and G4). The right eye cornea from each rat was cauterized using filter paper (3 mm), soaked in a solution of silver and potassium nitrates (3:1). Cauterization times were 10 (G1 and G4), or 20 seconds (G2 and G3). Cauterized corneas were washed with Ringer’s lactate solution. The filter paper was either removed before washing (G1 and G2), or kept on the corneas (G3 and G4). Corneas were photographed at multiple time points (2, 4, 6, 8, 11, 13, and 15 days after the procedure), and neovascularization parameters were assayed. Results: Neovascularization was observed in 66% of G1 corneas, and 100% of G2, G3, and G4 corneas. On day 15, G1 corneas showed smaller vascularized areas (12.63 ± 12.59%) compared to those in the G3 (41.95 ± 17.32%) and G4 (33 ± 11.74%) (P < 0.05) groups. Conclusions: The silver and potassium nitrate solution effectively induced corneal angiogenesis. The G2, G3, and G4 protocols showed excellent reproducibility, and induced vascularization in 100% of corneas.en
dc.description.affiliationOphthalmology Unit Department of Veterinary Clinical Medicine and Surgery Faculty of Agrarian and Veterinary Sciences Universidade Esatdual de São Paulo FCAV-UNESP
dc.description.affiliationUnespOphthalmology Unit Department of Veterinary Clinical Medicine and Surgery Faculty of Agrarian and Veterinary Sciences Universidade Esatdual de São Paulo FCAV-UNESP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2009/51773-4
dc.description.sponsorshipIdCNPq: 300833/2010-5
dc.format.extent607-616
dc.identifierhttp://dx.doi.org/10.1590/s0102-865020170080000002
dc.identifier.citationActa Cirurgica Brasileira, v. 32, n. 8, p. 607-616, 2017.
dc.identifier.doi10.1590/s0102-865020170080000002
dc.identifier.fileS0102-86502017000800607.pdf
dc.identifier.issn1678-2674
dc.identifier.issn0102-8650
dc.identifier.scieloS0102-86502017000800607
dc.identifier.scopus2-s2.0-85029455855
dc.identifier.urihttp://hdl.handle.net/11449/179172
dc.language.isoeng
dc.relation.ispartofActa Cirurgica Brasileira
dc.relation.ispartofsjr0,395
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCautery
dc.subjectCorneal neovascularization
dc.subjectRats
dc.subjectSilver
dc.titleCorneal angiogenesis based on different protocols of alkaline cauterization in murine modelsen
dc.typeArtigo

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