Identification, cloning, and characterization of a novel chitinase from leaf-cutting ant Atta sexdens: An enzyme with antifungal and insecticidal activity

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dc.contributor.authorMicocci, Kelli C. [UNESP]
dc.contributor.authorMoreira, Ariele C.
dc.contributor.authorSanchez, Amanda D.
dc.contributor.authorPettinatti, Jessica L.
dc.contributor.authorRocha, Marina C.
dc.contributor.authorDionizio, Bruna S.
dc.contributor.authorCorrea, Katia C.S.
dc.contributor.authorMalavazi, Iran
dc.contributor.authorWouters, Felipe C.
dc.contributor.authorBueno, Odair C. [UNESP]
dc.contributor.authorSouza, Dulce Helena F.
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2023-07-29T13:24:17Z
dc.date.available2023-07-29T13:24:17Z
dc.date.issued2023-01-01
dc.description.abstractChitinases are enzymes that degrade chitin, a polysaccharide found in the exoskeleton of insects, fungi, yeast, and internal structures of other vertebrates. Although chitinases isolated from bacteria, fungi and plants have been reported to have antifungal or insecticide activities, chitinases from insects with these activities have been seldomly reported. In this study, a leaf-cutting ant Atta sexdens DNA fragment containing 1623 base pairs was amplified and cloned into a vector to express the protein (AsChtII-C4B1) in Pichia pastoris. AsChtII-C4B1, which contains one catalytic domain and one carbohydrate-binding module (CBM), was secreted to the extracellular medium and purified by ammonium sulfate precipitation followed by nickel column chromatography. AsChtII-C4B1 showed maximum activity at pH 5.0 and 55 °C when tested against colloidal chitin substrate and maintained >60% of its maximal activity in different temperatures during 48 h. AsChtII-C4B1 decreased the survival of Spodoptera frugiperda larvae fed with an artificial diet that contained AsChtII-C4B1. Our results have indicated that AsChtII-C4B1 has a higher effect on larva-pupa than larva-larva molts. AsChtII-C4B1 activity targets more specifically the growth of filamentous fungus than yeast. This work describes, for the first time, the obtaining a recombinant chitinase from ants and the characterization of its insecticidal and antifungal activities.en
dc.description.affiliationCenter for the Study of Social Insects São Paulo State University “Julio de Mesquita Filho”, Rio Claro, SP
dc.description.affiliationDepartment of Physics Chemistry and Mathematics Federal University of São Carlos, SP
dc.description.affiliationDepartment of Chemistry Federal University of São Carlos, SP
dc.description.affiliationDepartment of Genetics and Evolution Federal University of São Carlos, SP
dc.description.affiliationUnespCenter for the Study of Social Insects São Paulo State University “Julio de Mesquita Filho”, Rio Claro, SP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2017/06198-8
dc.identifierhttp://dx.doi.org/10.1016/j.bbagen.2022.130249
dc.identifier.citationBiochimica et Biophysica Acta - General Subjects, v. 1867, n. 1, 2023.
dc.identifier.doi10.1016/j.bbagen.2022.130249
dc.identifier.issn1872-8006
dc.identifier.issn0304-4165
dc.identifier.scopus2-s2.0-85139724106
dc.identifier.urihttp://hdl.handle.net/11449/247732
dc.language.isoeng
dc.relation.ispartofBiochimica et Biophysica Acta - General Subjects
dc.sourceScopus
dc.subjectAntifungal activity
dc.subjectChitinase insect
dc.subjectInsecticide activity
dc.titleIdentification, cloning, and characterization of a novel chitinase from leaf-cutting ant Atta sexdens: An enzyme with antifungal and insecticidal activityen
dc.typeArtigo

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Rio Claro - CEIS - Centro de Estudos de Insetos Sociais