Effects of crude extracts of Agaricus blazei on DNA damage and on rat liver carcinogenesis induced by diethylnitrosamine

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dc.contributor.authorBarbisan, Luís Fernando [UNESP]
dc.contributor.authorScolastici, Clarissa [UNESP]
dc.contributor.authorMiyamoto, Maristela [UNESP]
dc.contributor.authorFarero Salvadori, Daisy Maria [UNESP]
dc.contributor.authorRibeiro, Lúcia Regina [UNESP]
dc.contributor.authorFerreira Da Eira, Augusto [UNESP]
dc.contributor.authorViana de Camargo, João Lauro [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Luterana do Brasil
dc.date.accessioned2014-05-27T11:20:59Z
dc.date.available2014-05-27T11:20:59Z
dc.date.issued2003-12-01
dc.description.abstractThe effects of crude extracts of the mushroom Agaricus blazei Murrill (Agaricaceae) on both DNA damage and placental form glutathione S-transferase (GST-P)-positive liver foci induced by diethylnitrosamine (DEN) were investigated. Six groups of adult male Wistar rats were used. For two weeks, animals of groups 3 to 6 were treated with three aqueous solutions of A. blazei (mean dry weight of solids being 1.2, 5.6, 11.5 and 11.5 mg/ml, respectively). After this period, groups 2 to 5 were given a single ip injection 200 mg/kg DEN and groups 1 and 6 were treated with 0.9% NaCl. All animals were subjected to 70% partial hepatectomy at week five and sacrificed 4, 24 and 48 h or 8 weeks after DEN or 0.9% NaCl treatments (10th week after the beginning of the experiment). The alkaline comet assay and GST-P-positive liver foci development were used to evaluate the influence of the mushroom extracts on liver cell DNA damage and on the initiation of liver carcinogenesis, respectively. Previous treatment with the highest concentration of A. blazei (11.5 mg/ml) significantly reduced DNA damage, indicating a protective effect against DEN-induced liver cytotoxicity/genotoxicity. However, the same dose of mushroom extract significantly increased the number of GST-P-positive liver foci.en
dc.description.affiliationDepartamento de Morfologia Instituto de Biologia UNESP, Botucatu, SP
dc.description.affiliationDepartamento de Patologia - TOXICAN Faculdade de Medicina UNESP, Botucatu, SP
dc.description.affiliationUniversidade Luterana do Brasil, Canoas, RS
dc.description.affiliationDepto. Prod. Vegetal, Mod. Cogumelos Faculdade de Ciencias Agronomicas UNESP, Botucatu, SP
dc.description.affiliationUnespDepartamento de Morfologia Instituto de Biologia UNESP, Botucatu, SP
dc.description.affiliationUnespDepartamento de Patologia - TOXICAN Faculdade de Medicina UNESP, Botucatu, SP
dc.description.affiliationUnespDepto. Prod. Vegetal, Mod. Cogumelos Faculdade de Ciencias Agronomicas UNESP, Botucatu, SP
dc.format.extent295-308
dc.identifierhttp://www.geneticsmr.com//year2003/vol2-3/pdf/gmr0056.pdf
dc.identifier.citationGenetics and Molecular Research, v. 2, n. 3, p. 295-308, 2003.
dc.identifier.file2-s2.0-2542421821.pdf
dc.identifier.issn1676-5680
dc.identifier.lattes3278528112652257
dc.identifier.scopus2-s2.0-2542421821
dc.identifier.urihttp://hdl.handle.net/11449/67543
dc.language.isoeng
dc.relation.ispartofGenetics and Molecular Research
dc.relation.ispartofsjr0,439
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAgaricux blazei
dc.subjectDNA damage
dc.subjectGST-P-positive liver foci
dc.subjectHepatocarcinogenesis
dc.subjectdiethylnitrosamine
dc.subjectglutathione transferase
dc.subjectliver protective agent
dc.subjectAgaricus blazei
dc.subjectanimal cell
dc.subjectanimal model
dc.subjectcomet assay
dc.subjectconcentration response
dc.subjectcontrolled study
dc.subjectdrug cytotoxicity
dc.subjectfungus
dc.subjectgenotoxicity
dc.subjectimmunohistochemistry
dc.subjectinflammation
dc.subjectliver carcinogenesis
dc.subjectliver cell
dc.subjectliver necrosis
dc.subjectnonhuman
dc.subjectpartial hepatectomy
dc.subjectrat
dc.subjecttail flick test
dc.subjectweight gain
dc.subjectAgaricaceae
dc.subjectAgaricus
dc.subjectAnimalia
dc.subjectBasidiomycota
dc.subjectFungi
dc.subjectRattus norvegicus
dc.titleEffects of crude extracts of Agaricus blazei on DNA damage and on rat liver carcinogenesis induced by diethylnitrosamineen
dc.typeArtigo
dcterms.licensehttp://www.geneticsmr.com/node/5
unesp.author.lattes3278528112652257
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agronômicas, Botucatupt
unesp.departmentProdução e Melhoramento Vegetal - FCApt

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