CHARACTERIZATION of RALSTONIA SOLANACEARUM STRAINS FROM BRAZIL USING MOLECULAR METHODS and PATHOGENICITY TESTS

Página do item simplificado
dc.contributor.authorRodrigues, L. M. R. [UNESP]
dc.contributor.authorDestefano, S. A. L.
dc.contributor.authorda Silva, M. J.
dc.contributor.authorCosta, G. G. L.
dc.contributor.authorMaringoni, Antonio Carlos [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInst Biol
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2014-05-20T13:21:23Z
dc.date.available2014-05-20T13:21:23Z
dc.date.issued2012-11-01
dc.description.abstractForty one Brazilian strains of Ralstonia solanacearum from ornamental plants (Begonia sp., Fuchsia sp., Oxalis sp., Pelargonium x hortorum and Tagetes sp.), eucalyptus (Eucalyptus sp.), Solanaceae (Nicotiana tabacum, Solanum jilo, S. lycopersicum, S. melon gena, S. tuberosum), banana (Musa sp.) and heliconia (Heliconia sp.), belonging to three different races and five biovars, were characterized by ERIC and BOX-PCR and classified in phylotypes by sequence analysis of the endoglucanase (Egl) and MutS genes. The virulence of 22 strains was evaluated on tomato cv. Petomech VF, potato cv. Agata and tobacco var. Burley. In the ERIC and BOX-PCR analysis, strains were separated into two main clusters showing high genetic diversity among them, with no correlation concerning race, biovar or geographical origin. Phylogenetic analysis based on sequences of part of the Egl and MutS genes revealed the predominance of phylotype II in Brazil. With exception of strains belonging to race 2, which were not included in the experiments, pathogenicity tests carried out with different strains of R. solanacearum showed high virulence on tomato and potato, and lower virulence on tobacco, showing no relationship with race, biovar or host plant from which the tested strain were originally isolated.en
dc.description.affiliationUNESP, Coll Agr Sci, Dept Plant Protect, BR-18603970 Botucatu, SP, Brazil
dc.description.affiliationInst Biol, Lab Plant Bacteriol, Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Ctr Mol Biol & Genet Engn, Campinas, SP, Brazil
dc.description.affiliationUnespUNESP, Coll Agr Sci, Dept Plant Protect, BR-18603970 Botucatu, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent505-516
dc.identifierhttp://dx.doi.org/10.4454/JPP.FA.2012.052
dc.identifier.citationJournal of Plant Pathology. Pisa: Edizioni Ets, v. 94, n. 3, p. 505-516, 2012.
dc.identifier.doi10.4454/JPP.FA.2012.052
dc.identifier.issn1125-4653
dc.identifier.lattes0464443742139470
dc.identifier.urihttp://hdl.handle.net/11449/6145
dc.identifier.wosWOS:000312039500004
dc.language.isoeng
dc.publisherEdizioni Ets
dc.relation.ispartofJournal of Plant Pathology
dc.relation.ispartofjcr0.944
dc.relation.ispartofsjr0,276
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectbacterial wilten
dc.subjectphylotypesen
dc.subjectvirulenceen
dc.subjectgenetic diversityen
dc.titleCHARACTERIZATION of RALSTONIA SOLANACEARUM STRAINS FROM BRAZIL USING MOLECULAR METHODS and PATHOGENICITY TESTSen
dc.typeArtigo
dcterms.rightsHolderEdizioni Ets
unesp.author.lattes0464443742139470[5]
unesp.author.orcid0000-0001-9108-4753[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agronômicas, Botucatupt
unesp.departmentProteção Vegetal - FCApt

Arquivos

Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Botucatu - FCA - Faculdade de Ciências Agronômicas