The presence of osteocalcin, osteopontin and reactive oxygen species-positive cells in pulp tissue after dental bleaching

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dc.contributor.authorBenetti, F. [UNESP]
dc.contributor.authorBriso, A. L.F. [UNESP]
dc.contributor.authorCarminatti, M. [UNESP]
dc.contributor.authorde Araújo Lopes, J. M. [UNESP]
dc.contributor.authorBarbosa, J. G. [UNESP]
dc.contributor.authorErvolino, E. [UNESP]
dc.contributor.authorGomes-Filho, J. E. [UNESP]
dc.contributor.authorCintra, L. T.A. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2019-10-06T15:28:10Z
dc.date.available2019-10-06T15:28:10Z
dc.date.issued2019-05-01
dc.description.abstractAim: To analyse the influence of H 2 O 2 on pulp repair through osteocalcin and osteopontin immunolabelling and in cellular defence by using the antireactive oxygen species (ROS) antibody. Methodology: The maxillary molars of 50 rats were treated with 35% H 2 O 2 (Ble groups) or placebo gel (control groups). At 0 h and 2, 7, 15 and 30 days (n = 10 hemimaxillae), the rats were killed and pulp tissue was evaluated using inflammation and immunolabelling scores (osteocalcin/osteopontin); ROS-positive cells were counted. Paired t-test and Wilcoxon signed-rank test were used (P < 0.05). Results: The Ble group had necrosis in the coronal pulp at 0 h and in the occlusal third of the coronal pulp at 2 days; at 7, 15 and 30 days, no inflammation was noted similar to the controls (P > 0.05). Osteocalcin was absent in the Ble at 0 h, moderate at 2 days and increased thereafter, differing from the controls at all two periods (P < 0.05). Osteopontin was higher principally at 7 and 15 days in Ble groups, but differing with control groups from 2 days after bleaching (P < 0.05). The Ble group had more ROS-positive cells in the pulp at 7 and 15 days (P < 0.05). Tertiary dentine was observed at 7 days, increasing thereafter (P < 0.05). Conclusions: Post-bleaching pulp repair was associated with increased osteocalcin over time. Osteopontin also participated in this process, and anti-ROS was involved in cellular defence against H 2 O 2 .en
dc.description.affiliationDepartment of Endodontics School of Dentistry São Paulo State University (Unesp)
dc.description.affiliationDepartment of Restorative Dentistry School of Dentistry São Paulo State University (Unesp)
dc.description.affiliationDepartment of Basic Science School of Dentistry São Paulo State University (Unesp)
dc.description.affiliationUnespDepartment of Endodontics School of Dentistry São Paulo State University (Unesp)
dc.description.affiliationUnespDepartment of Restorative Dentistry School of Dentistry São Paulo State University (Unesp)
dc.description.affiliationUnespDepartment of Basic Science School of Dentistry São Paulo State University (Unesp)
dc.format.extent665-675
dc.identifierhttp://dx.doi.org/10.1111/iej.13049
dc.identifier.citationInternational Endodontic Journal, v. 52, n. 5, p. 665-675, 2019.
dc.identifier.doi10.1111/iej.13049
dc.identifier.issn1365-2591
dc.identifier.issn0143-2885
dc.identifier.scopus2-s2.0-85058705733
dc.identifier.urihttp://hdl.handle.net/11449/187189
dc.language.isoeng
dc.relation.ispartofInternational Endodontic Journal
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectdental pulp
dc.subjecthydrogen peroxide
dc.subjectosteocalcin
dc.subjectosteopontin
dc.subjectreactive oxygen species
dc.subjecttertiary dentine
dc.titleThe presence of osteocalcin, osteopontin and reactive oxygen species-positive cells in pulp tissue after dental bleachingen
dc.typeArtigo
unesp.author.lattes5761956467234702[2]
unesp.author.orcid0000-0002-5459-353X[1]
unesp.author.orcid0000-0001-5994-2287[7]
unesp.author.orcid0000-0003-2348-7846[8]
unesp.author.orcid0000-0002-6126-1760[2]

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