Antiproliferative activity of standardized herbal phytopreparation from Asclepias subulata

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dc.contributor.authorGonzález Gutiérrez, Francisco Humberto
dc.contributor.authorRascón Valenzuela, Luisa Alondra
dc.contributor.authorMeneses Sagrero, Salvador Enrique
dc.contributor.authorDias-Silva, Marcelo J. [UNESP]
dc.contributor.authorValenzuela Antelo, Olivia
dc.contributor.authorVelazquez, Carlos
dc.contributor.authorVilegas, Wagner [UNESP]
dc.contributor.authorRobles Zepeda, Ramón Enrique
dc.contributor.institutionUniversidad de Sonora
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T16:10:32Z
dc.date.available2023-07-29T16:10:32Z
dc.date.issued2022-01-01
dc.description.abstractBackground: Several studies have shown that active compounds of Asclepias subulata (cardenolides) have antiproliferative effect on human cancer cells. Cardenolides isolated from A. subulata can be used as active chemical markers to elaborate phytopharmaceutical preparations. The aim of this work was to evaluate the antiproliferative effect of a standardized extract of the aerial parts, based on Asclepias subulata cardenolides. Methods: Four standardized extracts were prepared by HPLC-DAD depending on the concentration of calotropin and the antiproliferative activity was measured for the MTT assay, on the A549, MCF-7, HeLa, PC3 and ARPE cell lines. The concentrations of calotropin used for the standardization of the extracts were 10, 7.6, 5 and 1 mg/dL. Results: Standardization of the A. subulata extract based on calotropin at 7.6 mg/g dry weight was achieved and the antiproliferative activity was evaluated over A549, HeLa and MCF-7 cell lines, obtaining proliferation percentages of 3.8 to 13.4%. Conclusions: The standardized extracts of A. subulata at different concentrations of calotropin showed antiproliferative activity against all the cell lines evaluated. The greatest effect was observed against the HeLa cell line.en
dc.description.affiliationCiencias Químico Biológicas Universidad de Sonora, Sonora
dc.description.affiliationUniversidade Estadual Paulista (UNESP), Sao Paulo
dc.description.affiliationUnespUniversidade Estadual Paulista (UNESP), Sao Paulo
dc.identifierhttp://dx.doi.org/10.12688/f1000research.111181.2
dc.identifier.citationF1000Research, v. 11.
dc.identifier.doi10.12688/f1000research.111181.2
dc.identifier.issn1759-796X
dc.identifier.issn2046-1402
dc.identifier.scopus2-s2.0-85151827618
dc.identifier.urihttp://hdl.handle.net/11449/249835
dc.language.isoeng
dc.relation.ispartofF1000Research
dc.sourceScopus
dc.subjectAntiproliferative activity
dc.subjectAsclepias subulate
dc.subjectCalotropin
dc.subjectCardenolides
dc.subjectStandardized extract
dc.titleAntiproliferative activity of standardized herbal phytopreparation from Asclepias subulataen
dc.typeArtigo
unesp.author.orcid0000-0003-2846-3333[3]
unesp.author.orcid0000-0002-8988-5532[4]
unesp.author.orcid0000-0002-5279-4020[8]

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