Antibacterial Activity of Isobavachalcone (IBC) Is Associated with Membrane Disruption

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dc.contributor.authorde Assis, Leticia Ribeiro [UNESP]
dc.contributor.authorTheodoro, Reinaldo Dos Santos [UNESP]
dc.contributor.authorCosta, Maria Beatriz Silva [UNESP]
dc.contributor.authorNascentes, Julyanna Andrade Silva [UNESP]
dc.contributor.authorda Rocha, Miguel Divino [UNESP]
dc.contributor.authorBessa, Meliza Arantes de Souza
dc.contributor.authorMenezes, Ralciane de Paula
dc.contributor.authorDilarri, Guilherme [UNESP]
dc.contributor.authorHypolito, Giovane Böerner [UNESP]
dc.contributor.authorDos Santos, Vanessa Rodrigues [UNESP]
dc.contributor.authorDuque, Cristiane [UNESP]
dc.contributor.authorFerreira, Henrique [UNESP]
dc.contributor.authorMartins, Carlos Henrique Gomes
dc.contributor.authorRegasini, Luis Octavio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.date.accessioned2022-04-29T08:40:15Z
dc.date.available2022-04-29T08:40:15Z
dc.date.issued2022-03-01
dc.description.abstractIsobavachalcone (IBC) is a natural prenylated chalcone with a broad spectrum of pharmacological properties. In this work, we newly synthesized and investigated the antibacterial activity of IBC against Gram-positive, Gram-negative and mycobacterial species. IBC was active against Grampositive bacteria, mainly against Methicillin-Susceptible Staphylococcus aureus (MSSA) and MethicillinResistant Staphylococcus aureus (MRSA), with minimum inhibitory concentration (MIC) values of 1.56 and 3.12 µg/mL, respectively. On the other hand, IBC was not able to act against Gram-negative species (MIC > 400 µg/mL). IBC displayed activity against mycobacterial species (MIC = 64 µg/mL), including Mycobacterium tuberculosis, Mycobacterium avium and Mycobacterium kansasii. IBC was able to inhibit more than 50% of MSSA and MRSA biofilm formation at 0.78 µg/mL. Its antibiofilm activity was similar to vancomycin, which was active at 0.74 µg/mL. In order to study the mechanism of the action by fluorescence microscopy, the propidium iodide (PI) and SYTO9 fluorophores indicated that IBC disrupted the membrane of Bacillus subtilis. Toxicity assays using human keratinocytes (HaCaT cell line) showed that IBC did not have the capacity to reduce the cell viability. These results suggested that IBC is a promising antibacterial agent with an elucidated mode of action and potential applications as an antibacterial drug and a medical device coating.en
dc.description.affiliationDepartment of Chemistry and Environmental Sciences Institute of Biosciences Humanities and Exact Sciences São Paulo State University (Unesp), SP
dc.description.affiliationDepartment of Microbiology Institute of Biomedical Sciences Federal University of Uberlândia (UFU), MG
dc.description.affiliationDepartment of Biochemistry and Microbiology Institute of Biosciences São Paulo State University (Unesp), SP
dc.description.affiliationDepartment of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (Unesp), SP
dc.description.affiliationUnespDepartment of Chemistry and Environmental Sciences Institute of Biosciences Humanities and Exact Sciences São Paulo State University (Unesp), SP
dc.description.affiliationUnespDepartment of Biochemistry and Microbiology Institute of Biosciences São Paulo State University (Unesp), SP
dc.description.affiliationUnespDepartment of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (Unesp), SP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2014/18330-0
dc.description.sponsorshipIdFAPESP: 2018/15083-2
dc.description.sponsorshipIdCNPq: 306251/20167
dc.description.sponsorshipIdCNPq: 309957/2019-2
dc.description.sponsorshipIdCNPq: 429322/2018-6
dc.description.sponsorshipIdCNPq: 471129/2013-5
dc.identifierhttp://dx.doi.org/10.3390/membranes12030269
dc.identifier.citationMembranes, v. 12, n. 3, 2022.
dc.identifier.doi10.3390/membranes12030269
dc.identifier.issn2077-0375
dc.identifier.scopus2-s2.0-85125567508
dc.identifier.urihttp://hdl.handle.net/11449/230485
dc.language.isoeng
dc.relation.ispartofMembranes
dc.sourceScopus
dc.subjectAntibacterial
dc.subjectBiofilm
dc.subjectChalcone
dc.subjectMembrane
dc.subjectNatural product
dc.titleAntibacterial Activity of Isobavachalcone (IBC) Is Associated with Membrane Disruptionen
dc.typeArtigo

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Artigos - Bioquímica e Microbiologia - IBRC