LH Response (in vivo and in vitro) to an LHRH Agonist Administered to Domestic Male Cats

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dc.contributor.authorGenaro, G.
dc.contributor.authorLacerda Neto, J. C. [UNESP]
dc.contributor.authorRosa E Silva, A. A M
dc.contributor.institutionCtro. Univ. Barao de Maua
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:20:40Z
dc.date.available2014-05-27T11:20:40Z
dc.date.issued2003-07-01
dc.description.abstractWe investigated plasma luteinizing hormone (LH) concentration in domestic male cats challenged with Luteinizing Hormone Releasing Hormone Analog (LHRH-A) [des Gly10, (DTrp6)-LHRH ethylamide] that mediates the function of the hypothalamic-piruitary-gonadal axis (HPG). Plasma LH concentrations in cats treated daily with LHRH (10 μg/ 100 μl/kg/day, subcutaneously - sc) for 19 days (LHRH group) and in controls treated with saline (NaCl - 0.9%, same volume - SAL group) were chronically studied. LHRH administration (sc) for 15 days induced a significant fall (P < 0.05) in plasma LH concentrations during the chronic study. After the 15th day of treatment the groups were divided once more into animals treated with LHRH (10 μg/100 μl/kg) or saline (iv), and a time course study (300 min) was performed (acute study). Next, four groups of cats were compared in an acute study involving the sc/iv administration of SAL/SAL, SAL/LHRH, LHRH/SAL, and LHRH/LHRH. The responses of the SAL animals challenged by acute iv administration of LHRH (group SAL/LHRH) were significantly higher (P < 0.01) than those of animals treated with LHRH (sc) (group LHRH/LHRH). LH release was also significantly increased in the latter group (P < 0.05), although the effect was short lasting, being recorded only at the first observation (45 min). An in vitro study with the pituitaries was also performed on day 20. Mean (±SEM) LH concentrations in the culture medium containing pituitaries with LHRH (10-7 M) or saline were determined. In vitro analysis of these pituitaries demonstrated a significantly reduced response (P < 0.05) by animals treated sc with LHRH for 19 days. This study represents a source of data for the domestic cat going beyond its own physiology. Serving as a model, this animal provide important information for the study of reproductive physiology in other members of its family (Felidae), almost all of them threatened with extinction.en
dc.description.affiliationCtro. Univ. Barao de Maua, Ribeirão Preto, SP
dc.description.affiliationFaculdade Cie. Agrarias V. UNESP
dc.description.affiliationFaculdade Med. Ribeirão Preto USP
dc.description.affiliation, Caixa Postal - 390, Cep: 14001-970, Ribeirao Preto, SP
dc.description.affiliationUnespFaculdade Cie. Agrarias V. UNESP
dc.format.extent254-258
dc.identifierhttp://dx.doi.org/10.1076/apab.111.3.254.23456
dc.identifier.citationArchives of Physiology and Biochemistry, v. 111, n. 3, p. 254-258, 2003.
dc.identifier.doi10.1076/apab.111.3.254.23456
dc.identifier.issn1381-3455
dc.identifier.scopus2-s2.0-1642533673
dc.identifier.urihttp://hdl.handle.net/11449/67330
dc.language.isoeng
dc.relation.ispartofArchives of Physiology and Biochemistry
dc.relation.ispartofjcr1.806
dc.relation.ispartofsjr0,626
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCat
dc.subjectDomestic cat
dc.subjectFelids
dc.subjectLH
dc.subjectLHRH
dc.subjectReproduction
dc.subjectgonadorelin
dc.subjectgonadorelin derivative
dc.subjectgonadorelin[1-9] ethylamide[6 dextro alanine]
dc.subjectluteinizing hormone
dc.subjectsodium chloride
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectcat
dc.subjectcontrolled study
dc.subjectculture medium
dc.subjectdomestic animal
dc.subjecthypophysis
dc.subjectin vitro study
dc.subjectin vivo study
dc.subjectluteinizing hormone blood level
dc.subjectluteinizing hormone release
dc.subjectmale
dc.subjectnonhuman
dc.subjectphysiology
dc.subjectAnimals
dc.subjectAnimals, Domestic
dc.subjectCats
dc.subjectCulture Techniques
dc.subjectGonadotropin-Releasing Hormone
dc.subjectInjections, Subcutaneous
dc.subjectLuteinizing Hormone
dc.subjectMale
dc.subjectPituitary Gland, Anterior
dc.subjectTriptorelin
dc.subjectAnimalia
dc.subjectFelidae
dc.subjectFelis catus
dc.titleLH Response (in vivo and in vitro) to an LHRH Agonist Administered to Domestic Male Catsen
dc.typeArtigo
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