Synergetic antimicrobial effect of chlorin e6 and hydrogen peroxide on multi-species biofilms

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dc.contributor.authorNie, Min
dc.contributor.authorSilva, Rodrigo Costa e
dc.contributor.authorde Oliveira, Kleber Thiago
dc.contributor.authorBagnato, Vanderlei Salvador
dc.contributor.authorde Souza Rastelli, Alessandra Nara [UNESP]
dc.contributor.authorCrielaard, Wim
dc.contributor.authorYang, Jingmei
dc.contributor.authorDeng, Dong Mei
dc.contributor.institutionGuangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine
dc.contributor.institutionSichuan University
dc.contributor.institutionUniversity of Amsterdam and Vrije Universiteit Amsterdam
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-28T19:42:31Z
dc.date.available2022-04-28T19:42:31Z
dc.date.issued2021-01-01
dc.description.abstractAntimicrobial photodynamic therapy (aPDT) has been considered as a potential alternative to antibiotics for the treatment of biofilm infections. There is evidence that an additional H2O2 enhances the antimicrobial efficacy of aPDT. However, the minimum H2O2 concentration to achieve this synergistic effect is unclear. A saliva-derived multi-species biofilm was treated with the photosensitizer chlorin e6 (Ce6, 50 µM), H2O2 (0.3, 3.3, 33.3 mM), or their combination for 5 min, followed by no irradiation or irradiation at 15 J (cm2)−1 (λ = 450 nm or 660 nm), with or without oxygen. Biofilm viability and metabolic activity were evaluated. The combination of 33.3 mM H2O2 and Ce6-aPDT strongly enhanced antimicrobial efficacy compared with either component alone, irrespective of oxygen availability and irradiation wavelength. In particular, the combination resulted in a 6.6-log colony forming unit (CFU) reduction anaerobically under blue irradiation. This combination is a promising treatment for biofilm infections, especially those thriving in an anaerobic microenvironment.en
dc.description.affiliationDepartment of Periodontics Affiliated Stomatology Hospital of Guangzhou Medical University Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine
dc.description.affiliationState Key Laboratory of Oral Disease & National Clinical Research Center for Oral Diseases Department of Periodontics West China Hospital of Stomatology Sichuan University
dc.description.affiliationDepartment of Preventive Dentistry Academic Centre for Dentistry Amsterdam University of Amsterdam and Vrije Universiteit Amsterdam
dc.description.affiliationDepartment of Chemistry Federal University of São Carlos–UFSCar
dc.description.affiliationSão Carlos Institute of Physics University of São Paulo-USP São Carlos
dc.description.affiliationDepartment of Restorative Dentistry School of Dentistry São Paulo State University-UNESP
dc.description.affiliationUnespDepartment of Restorative Dentistry School of Dentistry São Paulo State University-UNESP
dc.format.extent656-665
dc.identifierhttp://dx.doi.org/10.1080/08927014.2021.1954169
dc.identifier.citationBiofouling, v. 37, n. 6, p. 656-665, 2021.
dc.identifier.doi10.1080/08927014.2021.1954169
dc.identifier.issn1029-2454
dc.identifier.issn0892-7014
dc.identifier.scopus2-s2.0-85111710150
dc.identifier.urihttp://hdl.handle.net/11449/222112
dc.language.isoeng
dc.relation.ispartofBiofouling
dc.sourceScopus
dc.subjectbiofilm
dc.subjectchlorin e6
dc.subjecthydrogen peroxide
dc.subjectoxygen
dc.subjectPhotodynamic therapy
dc.titleSynergetic antimicrobial effect of chlorin e6 and hydrogen peroxide on multi-species biofilmsen
dc.typeArtigo
unesp.author.orcid0000-0002-4318-9283[7]

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