Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate

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dc.contributor.authorFernandes, Gabriela Lopes [UNESP]
dc.contributor.authorVieira, Ana Paula Miranda [UNESP]
dc.contributor.authorDanelon, Marcelle [UNESP]
dc.contributor.authorEmerenciano, Nayara Gonçalves [UNESP]
dc.contributor.authorBerretta, Andresa Aparecida
dc.contributor.authorBuszinski, Andrei Felipe Moreira
dc.contributor.authorHori, Juliana Issa
dc.contributor.authorLima, Mikhael Haruo Fernandes de
dc.contributor.authorReis, Thaila Fernanda dos [UNESP]
dc.contributor.authorLima, Jessica Aparecida de
dc.contributor.authorDelbem, Alberto Carlos Botazzo [UNESP]
dc.contributor.authorSilva, Sónia Carina Morais da
dc.contributor.authorBarbosa, Debora Barros [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Ribeirão Preto—UNAERP
dc.contributor.institutionApis Flora Industrial and Comercial Ltd
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionNational Institute for Agrarian and Veterinary Research
dc.date.accessioned2023-07-29T13:27:47Z
dc.date.available2023-07-29T13:27:47Z
dc.date.issued2022-11-01
dc.description.abstractThis study investigated the anti-caries and anti-inflammatory effects of mouthwash formulations containing Punica granatum (pomegranate) peel extract (PPE), sodium-trimetaphosphate, and low concentrations of fluoride. PPE was characterized using high-performance liquid chromatography (ellagic acid and punicalagin). Total phenolics were quantified among formulations, and their stability was analyzed for 28 days. The formulation effects were evaluated as follows: (1) inorganic component concentration and reduced demineralization on bovine enamel blocks subjected to pH cycling; (2) anti-biofilm effect on dual-biofilms of Streptococcus mutans ATCC 25175 and Candida albicans ATCC 10231 treated for 1 and 10 min, respectively; and (3) cytotoxicity and production of inflammatory mediators (interleukin-6 and tumor necrosis factor-alpha). The formulation containing 3% PPE, 0.3% sodium-trimetaphosphate, and 225 ppm of fluoride resulted in a 34.5% surface hardness loss; a 13% (treated for 1 min) and 36% (treated for 10 min) biofilm reduction in S. mutans; a 26% (1 min) and 36% (10 min) biofilm reduction in C. albicans; absence of cytotoxicity; and anti-inflammatory activity confirmed by decreased interleukin-6 production in mouse macrophages. Thus, our results provide a promising prospect for the development of an alcohol-free commercial dental product with the health benefits of P. granatum that have been recognized for a millennium.en
dc.description.affiliationGraduate Program of Dental Science School of Dentistry Araçatuba São Paulo State University (UNESP), São Paulo
dc.description.affiliationSchool of Dentistry University of Ribeirão Preto—UNAERP, São Paulo
dc.description.affiliationDepartment of Restorative Dentistry School of Dentistry Araçatuba São Paulo State University (UNESP), São Paulo
dc.description.affiliationApis Flora Industrial and Comercial Ltd, São Paulo
dc.description.affiliationDepartment of Biochemistry and Immunology University of São Paulo Ribeirão Preto
dc.description.affiliationDepartment of Dental Materials and Prosthodontics School of Dentistry Araçatuba São Paulo State University (UNESP)
dc.description.affiliationNational Institute for Agrarian and Veterinary Research, Vairão
dc.description.affiliationUnespGraduate Program of Dental Science School of Dentistry Araçatuba São Paulo State University (UNESP), São Paulo
dc.description.affiliationUnespDepartment of Restorative Dentistry School of Dentistry Araçatuba São Paulo State University (UNESP), São Paulo
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics School of Dentistry Araçatuba São Paulo State University (UNESP)
dc.identifierhttp://dx.doi.org/10.3390/antibiotics11111477
dc.identifier.citationAntibiotics, v. 11, n. 11, 2022.
dc.identifier.doi10.3390/antibiotics11111477
dc.identifier.issn2079-6382
dc.identifier.scopus2-s2.0-85141747023
dc.identifier.urihttp://hdl.handle.net/11449/247858
dc.language.isoeng
dc.relation.ispartofAntibiotics
dc.sourceScopus
dc.subjectanti-inflammatory
dc.subjectantimicrobial
dc.subjectdental enamel
dc.subjectfluoride
dc.subjectpolyphosphates
dc.subjectPunica granatum
dc.titlePomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphateen
dc.typeArtigo
unesp.author.orcid0000-0003-2091-649X[3]
unesp.author.orcid0000-0002-5421-3728[7]
unesp.author.orcid0000-0002-8159-4853[11]
unesp.author.orcid0000-0003-0000-7387[12]
unesp.author.orcid0000-0002-3623-5093[13]

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Artigos - Odontologia Restauradora - FOA
Artigos - Materiais Odontológicos e Prótese - FOA