Methodology for fast evaluation of Bacillus thuringiensis crystal protein content

Página do item simplificado
dc.contributor.authorAlves, LMC
dc.contributor.authorLemos, MVF
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-10T18:00:53Z
dc.date.available2020-12-10T18:00:53Z
dc.date.issued2000-07-01
dc.description.abstractThe development of the production and use of Bacillus thuringiensis Brazil at a commercial scale faces certain difficulties, among them the establishment of efficient methodologies for the quantitation of toxic products to be commercialized. Presently, the amount of toxin is given in percentage by analyzing the samples total protein content. Such methodology however, does not measure the actual amount of active protein present in the product, since most strains express different endotoxin genes and might even produce b-toxin. Since the various types of toxins exhibit different antigenic characteristics, this work has as objective the utilization of fast immunological techniques to quantify the level of crystal protein. Crystal protein produced by a subspecies of Bacillus thuringiensis var, israelensis was purified by ultracentrifugation and utilized to immunize rabbits and to produce hiperimmune sera. Such sera were latter used to evaluate the level of proteins on commercial bioinsecticide and on laboratory cultures of B. thuringiensis through the immunodot technique. The results were obtained by comparison of data obtained from reactions with known concentrations of crystal protein permitting to evaluate the level of such protein on various materials.en
dc.description.affiliationUNESP, Fac Ciencias Agr & Vet, Dept Biol Aplicada & Agropecuaria, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationUNESP, Fac Ciencias Agr & Vet, Dept Tecnol, Jaboticabal, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Ciencias Agr & Vet, Dept Biol Aplicada & Agropecuaria, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Ciencias Agr & Vet, Dept Tecnol, Jaboticabal, SP, Brazil
dc.format.extent216-219
dc.identifier.citationBrazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 31, n. 3, p. 216-219, 2000.
dc.identifier.issn1517-8382
dc.identifier.urihttp://hdl.handle.net/11449/195707
dc.identifier.wosWOS:000167680500012
dc.language.isoeng
dc.publisherSoc Brasileira Microbiologia
dc.relation.ispartofBrazilian Journal Of Microbiology
dc.sourceWeb of Science
dc.subjectBacillus thuringiensis
dc.subjectcrystal protein
dc.subjectimmunological methods
dc.titleMethodology for fast evaluation of Bacillus thuringiensis crystal protein contenten
dc.typeArtigo
dcterms.rightsHolderSoc Brasileira Microbiologia
unesp.departmentBiologia - FCAVpt
unesp.departmentTecnologia - FCAVpt

Arquivos

Coleções

Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias