Uncovering the molecular organization of unusual highly scattered 5S rDNA: The case of Chariesterus armatus (Heteroptera)

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dc.contributor.authorBardella, Vanessa Bellini
dc.contributor.authorCabral-de-Mello, Diogo Cavalcanti
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:23:56Z
dc.date.available2018-12-11T17:23:56Z
dc.date.issued2018-03-10
dc.description.abstractOne cluster of 5S rDNA per haploid genome is the most common pattern among Heteroptera. However, in Chariesterus armatus, highly scattered signals were noticed. We isolated and characterized the entire 5S rDNA unit of C. armatus aiming to a deeper knowledge of molecular organization of the 5S rDNA among Heteroptera and to understand possible causes and consequences of 5S rDNA chromosomal spreading. For a comparative analysis, we performed the same approach in Holymenia histrio with 5S rDNA restricted to one bivalent. Multiple 5S rDNA variants were observed in both species, though they were more variable in C. armatus, with some of variants corresponding to pseudogenes. These pseudogenes suggest birth-and-death mechanism, though homogenization was also observed (concerted evolution), indicating evolution through mixed model. Association between transposable elements and 5S rDNA was not observed, suggesting spreading of 5S rDNA through other mechanisms, like ectopic recombination. Scattered organization is a rare example for 5S rDNA, and such organization in C. armatus genome could have led to the high diversification of sequences favoring their pseudogenization.en
dc.description.affiliationDepartamento de Biologia, Instituto de Biociências/IB, UNESP - Universidade Estadual Paulista, Rio Claro, Brazil
dc.description.affiliationDepartamento de Biologia, Instituto de Biociências/IB, UNESP - Universidade Estadual Paulista, Rio Claro, Brazil. Electronic address: mellodc@rc.unesp.br
dc.format.extent153-158
dc.identifierhttp://dx.doi.org/10.1016/j.gene.2017.12.030
dc.identifier.citationGene, v. 646, p. 153-158.
dc.identifier.doi10.1016/j.gene.2017.12.030
dc.identifier.file2-s2.0-85041262627.pdf
dc.identifier.issn1879-0038
dc.identifier.scopus2-s2.0-85041262627
dc.identifier.urihttp://hdl.handle.net/11449/177086
dc.language.isoeng
dc.relation.ispartofGene
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBirth-and-death
dc.subjectConcerted evolution
dc.subjectCoreidae
dc.subjectFish
dc.subjectMultigene family
dc.titleUncovering the molecular organization of unusual highly scattered 5S rDNA: The case of Chariesterus armatus (Heteroptera)en
dc.typeArtigo

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