Derivation and differentiation of canine ovarian mesenchymal stem cells

Página do item simplificado
dc.contributor.authorHill, Amanda B. T. [UNESP]
dc.contributor.authorHill, Jonathan E. B. T. [UNESP]
dc.contributor.authorBressan, Fabiana F.
dc.contributor.authorMiglino, Maria Angélica
dc.contributor.authorGarcia, Joaquim M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversité de Montréal
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2019-10-06T15:29:04Z
dc.date.available2019-10-06T15:29:04Z
dc.date.issued2018-12-01
dc.description.abstractInterest in mesenchymal stem cells (MSCs) has increased over the past decade due to their ease of isolation, expansion, and culture. Recently, studies have demonstrated the wide differentiation capacity that these cells possess. The ovary represents a promising candidate for cell-based therapies due to the fact that it is rich in MSCs and that it is frequently discarded after ovariectomy surgeries as biological waste. This article describes procedures for the isolation, expansion, and differentiation of MSCs derived from the canine ovary, without the necessity of cell-sorting techniques. This protocol represents an important tool for regenerative medicine because of the broad applicability of these highly differentiable cells in clinical trials and therapeutic uses.en
dc.description.affiliationThe School of Agricultural and Veterinary Studies (FCAV) Jaboticabal São Paulo State University (UNESP)
dc.description.affiliationCRRF Université de Montréal
dc.description.affiliationFZEA University of São Paulo
dc.description.affiliationFMVZ University of São Paulo
dc.description.affiliationUnespThe School of Agricultural and Veterinary Studies (FCAV) Jaboticabal São Paulo State University (UNESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2013/14293-0
dc.identifierhttp://dx.doi.org/10.3791/58163
dc.identifier.citationJournal of Visualized Experiments, v. 2018, n. 142, 2018.
dc.identifier.doi10.3791/58163
dc.identifier.issn1940-087X
dc.identifier.scopus2-s2.0-85059260873
dc.identifier.urihttp://hdl.handle.net/11449/187215
dc.language.isoeng
dc.relation.ispartofJournal of Visualized Experiments
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectDevelopmental Biology
dc.subjectDifferentiation
dc.subjectDog
dc.subjectHomogeneous population
dc.subjectIsolation
dc.subjectIssue 142
dc.subjectMesenchymal stem cell
dc.subjectOvary
dc.subjectPlastic adherence
dc.subjectStem cells
dc.titleDerivation and differentiation of canine ovarian mesenchymal stem cellsen
dc.typeArtigo

Arquivos

Coleções

Artigos