Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood

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dc.contributor.authorMárquez, Augusto
dc.contributor.authorSantos, Moliria V.
dc.contributor.authorGuirado, Gonzalo
dc.contributor.authorMoreno, Alex
dc.contributor.authorAznar-Cervantes, Salvador D.
dc.contributor.authorCenis, Jose Luis
dc.contributor.authorSantagneli, Silvia H. [UNESP]
dc.contributor.authorDomínguez, Carlos
dc.contributor.authorOmenetto, Fiorenzo G.
dc.contributor.authorMuñoz-Berbel, Xavier
dc.contributor.institutionCSIC)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversitat Autònoma de Barcelona Bellaterra (Barcelona)
dc.contributor.institutionInstituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionTufts University
dc.date.accessioned2021-06-25T10:22:39Z
dc.date.available2021-06-25T10:22:39Z
dc.date.issued2021-02-07
dc.description.abstractIn optical biosensing, silk fibroin (SF) appears as a promising alternative where other materials, such as paper, find limitations. Besides its excellent optical properties and unmet capacity to stabilize biomacromolecules, SF in test strips exhibits additional functions,i.e.capillary pumping activity of 1.5 mm s−1, capacity to filter blood cells thanks to its small, but tuneable, porosity and enhanced biosensing sensitivity. The bulk functionalization of SF with the enzymes glucose oxidase and peroxidase and the mediator ABTS produces colourless and transparent SF films that respond to blood glucose increasing 2.5 times the sensitivity of conventional ABTS-based assays. This enhanced sensitivity results from the formation of SF-ABTS complexes, where SF becomes part of the bioassay. Additionally, SF films triple the durability of most stable cellulose-based sensors. Although demonstrated for glucose, SF microfluidic test strips may incorporate other optical bioassays,e.g.immunoassays, with the aim of transferring them from central laboratories to the place of patient's care.en
dc.description.affiliationInstituto de Microelectrónica de Barcelona (IMB-CNM CSIC)
dc.description.affiliationSão Carlos Institute of Physics University of São Paulo
dc.description.affiliationDepartament de Química Universitat Autònoma de Barcelona Bellaterra (Barcelona)
dc.description.affiliationInstituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA)
dc.description.affiliationInstitute of Chemistry São Paulo State University (UNESP)
dc.description.affiliationSilklab Department of Biomedical Engineering Tufts University
dc.description.affiliationDepartment of Electrical and Computer Engineering Tufts University
dc.description.affiliationDepartment of Physics Tufts University
dc.description.affiliationLaboratory for Living Devices Tufts University
dc.description.affiliationUnespInstitute of Chemistry São Paulo State University (UNESP)
dc.format.extent608-615
dc.identifierhttp://dx.doi.org/10.1039/d0lc00702a
dc.identifier.citationLab on a Chip, v. 21, n. 3, p. 608-615, 2021.
dc.identifier.doi10.1039/d0lc00702a
dc.identifier.issn1473-0189
dc.identifier.issn1473-0197
dc.identifier.scopus2-s2.0-85100812402
dc.identifier.urihttp://hdl.handle.net/11449/205874
dc.language.isoeng
dc.relation.ispartofLab on a Chip
dc.sourceScopus
dc.titleNanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blooden
dc.typeArtigo

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