Proteome profiling of methyl jasmonate elicitation of Maytenus ilicifolia in vitro roots reveals insights into sesquiterpene pyridine alkaloids

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dc.contributor.authorSantos, Vânia A. F. F. M. dos [UNESP]
dc.contributor.authorCoppede, Juliana da Silva
dc.contributor.authorDias, Nathalia Batista
dc.contributor.authorPereira, Ana Maria Soares
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.contributor.authorFurlan, Maysa [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de Ribeirão Preto (UNAERP)
dc.contributor.institutionUniversidad de La Frontera (UFRO)
dc.date.accessioned2023-07-29T15:13:19Z
dc.date.available2023-07-29T15:13:19Z
dc.date.issued2022-12-01
dc.description.abstractMaytenus ilicifolia (Celastraceae) is an important medicinal plant widely used in Brazil and a rich source of sesquiterpene pyridine alkaloids (SPAs) with relevant biological activities. They exhibit great chemical diversity and high structural complexity, and show accumulation in very low concentrations. Thus, to determine whether in vitro cultured adventitious roots of M. ilicifolia could provide alternative sources for the production of these compounds, the accumulation of SPAs ilicifoliunine A and aquifoliunine E-I, as well as the time of production and the content of these alkaloids were evaluated using methyl jasmonate (MeJA) as an elicitor. The results showed the accumulation ilicifoliunine A and aquifoliunine E-I and an increase in the contents of these alkaloids when elicited with MeJA at 7 and 28 days, respectively. To characterize and identify the proteins present in the enzymatic system and contribute to the understanding of the metabolic pathways involved in the biosynthesis of SPAs, was carried out in vitro proteomic profiling of adventitious roots elicited and non-elicited with MeJA. The shotgun proteomic analyses led to the identification of important proteins involved in the biosynthetic pathway of SPAs, including germacrene A synthase, β-eudesmol synthase, and L-aspartate oxidase. Additionally, many proteins that participate in the biosynthesis of other alkaloids (e.g., tropane, pyrrolidine, terpenoid indole, and isoquinoline) and phenylpropanoids have been identified. In conclusion, the study report for the first time, proteins involved in the biosynthetic pathway of SPAs by using the adventitious roots in vitro system of M. ilicifolia and a shotgun proteomic approach.en
dc.description.affiliationInstituto de Química Universidade Estadual Paulista (UNESP)
dc.description.affiliationDepartamento de Biotecnologia Vegetal Universidade de Ribeirão Preto (UNAERP)
dc.description.affiliationScientific and Technological Bioresource Nucleus BIOREN-UFRO Universidad de La Frontera (UFRO)
dc.description.affiliationInstituto de Biociências Departamento de Biologia Geral e Aplicada Universidade Estadual Paulista (UNESP)
dc.description.affiliationUnespInstituto de Química Universidade Estadual Paulista (UNESP)
dc.description.affiliationUnespInstituto de Biociências Departamento de Biologia Geral e Aplicada Universidade Estadual Paulista (UNESP)
dc.format.extent551-563
dc.identifierhttp://dx.doi.org/10.1007/s11240-022-02371-9
dc.identifier.citationPlant Cell, Tissue and Organ Culture, v. 151, n. 3, p. 551-563, 2022.
dc.identifier.doi10.1007/s11240-022-02371-9
dc.identifier.issn1573-5044
dc.identifier.issn0167-6857
dc.identifier.scopus2-s2.0-85141345277
dc.identifier.urihttp://hdl.handle.net/11449/249338
dc.language.isoeng
dc.relation.ispartofPlant Cell, Tissue and Organ Culture
dc.sourceScopus
dc.subjectCelastraceae
dc.subjectIn vitro adventitious roots
dc.subjectMaytenus ilicifolia
dc.subjectMethyl jasmonate
dc.subjectSesquiterpene pyridine alkaloids
dc.subjectShotgun proteomic
dc.titleProteome profiling of methyl jasmonate elicitation of Maytenus ilicifolia in vitro roots reveals insights into sesquiterpene pyridine alkaloidsen
dc.typeArtigo
unesp.author.orcid0000-0002-2645-8031[1]
unesp.author.orcid0000-0002-4197-9927[2]
unesp.author.orcid0000-0002-5902-634X[3]
unesp.author.orcid0000-0002-3478-4718[4]
unesp.author.orcid0000-0002-7363-8211[5]
unesp.author.orcid0000-0002-8434-5238[6]

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