The First Anti-Snakebite and Hepatoprotective Characterization of a Trypsin Kunitz-like Inhibitor (EcTI) from the Plant Enterolobium contortisiliquum: a Case of Two Soul Mates Meeting

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dc.contributor.authorCosta, Caroline R. C. [UNESP]
dc.contributor.authorBelchor, Mariana N. [UNESP]
dc.contributor.authorRoggero, Airam [UNESP]
dc.contributor.authorMoraes, Laila L. [UNESP]
dc.contributor.authorSamelo, Ricardo [UNESP]
dc.contributor.authorAnnunciato, Isabelly [UNESP]
dc.contributor.authorBonturi, Camila R.
dc.contributor.authorOliva, Maria L. V.
dc.contributor.authorSousa, Sergio F.
dc.contributor.authorde Oliveira, Marcos A. [UNESP]
dc.contributor.authorToyama, Marcos H. [UNESP]
dc.contributor.institutionUniversidade Federal do ABC (UFABC)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionPorto University
dc.date.accessioned2023-07-29T16:12:44Z
dc.date.available2023-07-29T16:12:44Z
dc.date.issued2023-04-01
dc.description.abstractSnake venom serine protease (SVSP) interferes with the regulation and control of important biological reactions in homeostasis and can be classified as an activator of the fibrinolytic system and platelet aggregation. Our group has recently isolated a new serine protease from Crotalus durissus terrificus total venom (Cdtsp-2). This protein exhibits edematogenic capacity and myotoxic activity. A Kunitz-like EcTI inhibitor protein with a molecular mass of 20 kDa was isolated from Enterolobium contortisiliquum and showed high trypsin inhibition. Thus, the objective of this work is to verify the possible inhibition of the pharmacological activities of Cdtsp-2 by the Kutinz-type inhibitor EcTI. To isolate Cdtsp-2 from total C. d. terrificus venom, we used three-step chromatographic HPLC. Using the mice paw edema model, we observed an edematogenic effect, myotoxicity and hepatotoxicity caused by Cdtsp-2. In vitro and in vivo experiments showed that the alterations in hemostasis caused by Cdtsp-2 are crucial for the development of marked hepatotoxicity and that EcTI significantly inhibits the enzymatic and pharmacological activities of Cdtsp-2. Kunitz-like inhibitor may be a viable alternative for the development of ancillary treatments against the biological activities of venoms.en
dc.description.affiliationCenter of Natural and Human Sciences Federal University of ABC (UFABC), SP
dc.description.affiliationUniversity of São Paulo State (UNESP), Biosciences Institute of Paulista Coast Campus (IB/CLP), SP
dc.description.affiliationNational Institute of Pharmacology (INFAR) Federal University of São Paulo (UNIFESP), SP
dc.description.affiliationUnit of Applied Biomolecular Sciences (UCIBIO) REQUIMTE-BioSIM-Medicine Faculty Porto University
dc.description.affiliationUnespUniversity of São Paulo State (UNESP), Biosciences Institute of Paulista Coast Campus (IB/CLP), SP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2003/10516-2
dc.description.sponsorshipIdFAPESP: 2017/20291-0
dc.description.sponsorshipIdCNPq: 309271/2022-3
dc.description.sponsorshipIdFAPESP: p2017/19942-7
dc.identifierhttp://dx.doi.org/10.3390/ph16040632
dc.identifier.citationPharmaceuticals, v. 16, n. 4, 2023.
dc.identifier.doi10.3390/ph16040632
dc.identifier.issn1424-8247
dc.identifier.scopus2-s2.0-85154575295
dc.identifier.urihttp://hdl.handle.net/11449/249914
dc.language.isoeng
dc.relation.ispartofPharmaceuticals
dc.sourceScopus
dc.subjectCdtsp-2
dc.subjectCrotalus durissus terrificus
dc.subjectKunitz-type inhibitor
dc.subjectserine protease
dc.titleThe First Anti-Snakebite and Hepatoprotective Characterization of a Trypsin Kunitz-like Inhibitor (EcTI) from the Plant Enterolobium contortisiliquum: a Case of Two Soul Mates Meetingen
dc.typeArtigo
unesp.author.orcid0000-0003-4546-8013[3]
unesp.author.orcid0000-0001-7884-6768[6]
unesp.author.orcid0000-0001-9324-8424[7]
unesp.author.orcid0000-0001-7346-6328[8]
unesp.author.orcid0000-0002-6560-5284[9]

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