CO2 exposure enhances Fos expression in hypothalamic neurons in rats during the light and dark phases of the diurnal cycle

Dias, Mirela Barros; Silva, Eliandra Nunes da; Horta-Júnior, José de Anchieta Castro [UNESP]; Gargaglioni, Luciane Helena [UNESP]

CO2 exposure enhances Fos expression in hypothalamic neurons in rats during the light and dark phases of the diurnal cycle

dc.contributor.author	Dias, Mirela Barros
dc.contributor.author	Silva, Eliandra Nunes da
dc.contributor.author	Horta-Júnior, José de Anchieta Castro [UNESP]
dc.contributor.author	Gargaglioni, Luciane Helena [UNESP]
dc.contributor.institution	Universidade Estadual Paulista (Unesp)
dc.date.accessioned	2022-04-27T13:13:47Z
dc.date.available	2022-04-27T13:13:47Z
dc.date.issued	2022-04-23
dc.description	Orexinergic (OX) neurons in the lateral hypothalamus (LH), perifornical area (PFA) and dorsomedial hypothalamus (DMH) play a role in the hypercapnic ventilatory response, presumably through direct inputs to central pattern generator sites and/or through interactions with other chemosensitive regions. OX neurons can produce and release orexins, excitatory neuropeptides involved in many functions, including physiological responses to changes in CO2/pH. Thus, in the present study, we tested the hypothesis that different nuclei (LH, PFA and DMH) where the orexinergic neurons are located, show distinct activation by CO2 during the light-dark cycle phases. For this purpose, we evaluated the Fos and OXA expression by immunohistochemistry to identify neurons that co-localize Fos+OXA in the LH, LPeF, MPeF and DMH in the light-inactive and dark-active phase in Wistar rats subjected to 3 hours of normocapnia or hypercapnia (7% CO2). Quantitative analyses of immunoreactive neurons show that hypercapnia caused an increase in the number of neurons expressing Fos in the LH, LPeF, MPeF and DMH in the light and dark phases. In addition, the number of Fos+OXA neurons increased in both phases of the diurnal cycle in the LPeF and DMH, whereas in the MPeF, this increase was observed exclusively in the light phase. Thus, we suggest that OX neurons are selectivity activated by hypercapnia throughout the diurnal cycle, reinforcing the differential role of nuclei in the hypothalamus during central chemosensitivity.	pt
dc.description.sponsorship	Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship	Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipId	FAPESP: 2013/04216-8
dc.description.sponsorshipId	FAPESP: 2016/13136-6
dc.description.sponsorshipId	CAPES: 001
dc.description.sponsorshipId	CAPES: 88887.194785/2018-00
dc.format	Digital	pt
dc.identifier.lattes	3203493045928389
dc.identifier.lattes	2577584360099382
dc.identifier.lattes	5850453468994497
dc.identifier.orcid	0000-0002-4084-3139
dc.identifier.orcid	0000-0003-3639-9861
dc.identifier.orcid	0000-0003-4097-5286
dc.identifier.uri	http://hdl.handle.net/11449/218033
dc.language.iso	eng
dc.rights.accessRights	Acesso aberto
dc.rights.holder	Mirela Barros Dias	pt
dc.subject	Breathing	pt
dc.subject	Chemoreceptors	pt
dc.subject	Hypothalamus	pt
dc.title	CO2 exposure enhances Fos expression in hypothalamic neurons in rats during the light and dark phases of the diurnal cycle	pt
dc.type	Dado de pesquisa
unesp.campus	Universidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatu	pt