Colorimetric assay of ethanol using alcohol dehydrogenase from dry baker's yeast

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dc.contributor.authorZanon, Juliana P.
dc.contributor.authorPeres, Maristela F. S.
dc.contributor.authorGattas, Edwil Aparecida de Lucca [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:27:25Z
dc.date.available2014-05-20T15:27:25Z
dc.date.issued2007-02-05
dc.description.abstractAlcohol dehydrogenases (ADHs) are oxidoreductases present in animal tissues, plants, and microorganisms. These enzymes attract major scientific interest for the evolutionary perspectives, afforded by their wide occurrence in nature, and for their use in synthesis, thanks to their broad substrate specificity and stereoselectivity. In the present study, the standardization of the activity of the alcohol dehydrogenase from baker's yeast was accomplished, and the pH and temperature stability showed, that the enzyme presented a high stability to pH 6.0-7.0 and the thermal stability were completely maintained up to 50 degrees C during 1 h. The assays of ethanol (detection range 1-5 mM or 4.6 x 10(-2) to 23.0 x 10(-2) g/L) in different samples in alcoholic beverages, presented a maximum deviation of only 7.2%. The standard curve and the analytic curve of this method meet the conditions of precision, sensitivity, simplicity, and low cost, required for a useable analytical method. (c) 2006 Elsevier B.V. All rights reserved.en
dc.description.affiliationSão Paulo State Univ, Sch Pharmaceut Sci, Dept Food & Nutr, BR-14801902 São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Sch Pharmaceut Sci, Dept Food & Nutr, BR-14801902 São Paulo, Brazil
dc.format.extent466-470
dc.identifierhttp://dx.doi.org/10.1016/j.enzmictec.2006.07.029
dc.identifier.citationEnzyme and Microbial Technology. New York: Elsevier B.V., v. 40, n. 3, p. 466-470, 2007.
dc.identifier.doi10.1016/j.enzmictec.2006.07.029
dc.identifier.issn0141-0229
dc.identifier.lattes4006598610021833
dc.identifier.urihttp://hdl.handle.net/11449/37401
dc.identifier.wosWOS:000244072500017
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofEnzyme and Microbial Technology
dc.relation.ispartofjcr2.932
dc.relation.ispartofsjr0,754
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectalcohol dehydrogenasept
dc.subjectcolorimetric assay of ethanolpt
dc.subjectbaker's yeastpt
dc.titleColorimetric assay of ethanol using alcohol dehydrogenase from dry baker's yeasten
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes4006598610021833[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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Artigos - Alimentos e Nutrição - FCFAR