Electrospun Nanofibrous Poly (Lactic Acid)/Titanium Dioxide Nanocomposite Membranes for Cutaneous Scar Minimization

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dc.contributor.authorMarsi, Teresa C. O.
dc.contributor.authorRicci, Ritchelli
dc.contributor.authorToniato, Tatiane
dc.contributor.authorVasconcellos, Luana M. R. [UNESP]
dc.contributor.authorVaz Elias, Conceicao de Maria
dc.contributor.authorSilva, Andre D. R.
dc.contributor.authorFurtado, Andre S. A.
dc.contributor.authorMagalhaes, Leila S. S. M.
dc.contributor.authorSilva-Filho, Edson C.
dc.contributor.authorMarciano, Fernanda R.
dc.contributor.authorZille, Andrea
dc.contributor.authorWebster, Thomas J.
dc.contributor.authorLobo, Anderson O.
dc.contributor.institutionUniv Vale Do Paraiba
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionBrasil Univ
dc.contributor.institutionAir Force Acad
dc.contributor.institutionUFPI Fed Univ Piaui
dc.contributor.institutionUniv Fed Piaui
dc.contributor.institutionUniv Minho
dc.contributor.institutionNortheastern Univ
dc.date.accessioned2020-12-10T17:04:29Z
dc.date.available2020-12-10T17:04:29Z
dc.date.issued2019-12-20
dc.description.abstractPoly (lactic acid) (PLA) has been increasingly used in cutaneous tissue engineering due to its low cost, ease of handling, biodegradability, and biocompatibility, as well as its ability to form composites. However, these polymers possess a structure with nanoporous that mimic the cellular environment. In this study, nanocomposites are prepared using PLA and titanium dioxide (TiO2) (10 and 35%-w/w) nanoparticles that also function as an active anti-scarring agent. The nanocomposites were prepared using an electrospinning technique. Three different solutions were prepared as follows: PLA, 10% PLA/TiO2, and 35% PLA/TiO2 (w/w%). Electrospun PLA and PLA/TiO2 nanocomposites were characterized morphologically, structurally, and chemically using electron scanning microscopy, transmission electron microscopy, goniometry, and X-ray diffraction. L929 fibroblast cells were used for in vitro tests. The cytotoxic effect was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Versicam (VCAN), biglicam (BIG), interleukin-6 (IL6), interleukin-10 (IL-10), and type-1 collagen (COL1A1) genes were evaluated by RT-qPCR. In vivo tests using Wistar rats were conducted for up to 15 days. Nanofibrous fibers were obtained for all groups that did not contain residual solvents. No cytotoxic effects were observed for up to 168 h. The genes expressed showed the highest values of versican and collagen-1 (p < 0.05) for PLA/TiO2 nanocomposite scaffolds when compared to the control group (cells). Histological images showed that PLA at 10 and 35% w/w led to a discrete inflammatory infiltration and expression of many newly formed vessels, indicating increased metabolic activity of this tissue. To summarize, this study supported the potential of PLA/TiO2 nanocomposites ability to reduce cutaneous scarring in scaffolds.en
dc.description.affiliationUniv Vale Do Paraiba, Inst Res & Dev, Sao Jose Dos Campos, Brazil
dc.description.affiliationSao Paulo State Univ, Dept Biosci & Oral Diag, Inst Sci & Technol, Sao Paulo, SP, Brazil
dc.description.affiliationBrasil Univ, Sci & Technol Inst, Sao Paulo, SP, Brazil
dc.description.affiliationAir Force Acad, Brazilian Air Force, Pirassununga, Brazil
dc.description.affiliationUFPI Fed Univ Piaui, LIMAV Interdisciplinary Lab Adv Mat, Mat Sci & Engn Grad Program, Teresina, Brazil
dc.description.affiliationUniv Fed Piaui, Dept Phys, Teresina, Brazil
dc.description.affiliationUniv Minho, Ctr Text Sci & Technol, Dept Text Engn, Guimaraes, Portugal
dc.description.affiliationNortheastern Univ, Dept Chem Engn, Boston, MA 02115 USA
dc.description.affiliationUnespSao Paulo State Univ, Dept Biosci & Oral Diag, Inst Sci & Technol, Sao Paulo, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFCT
dc.description.sponsorshipInvestigator FCT Research contract
dc.description.sponsorshipFEDER
dc.description.sponsorshipPOCI
dc.description.sponsorshipIdCNPq: 303752/2017-3
dc.description.sponsorshipIdCNPq: 404683/2018-5
dc.description.sponsorshipIdCNPq: 304133/2017-5
dc.description.sponsorshipIdCNPq: 424163/2016-0
dc.description.sponsorshipIdFCT: UID/CTM/00264/2019
dc.description.sponsorshipIdFCT: PTDC/CTM-TEX/28295/2017
dc.description.sponsorshipIdInvestigator FCT Research contract: IF/00071/2015
dc.description.sponsorshipIdFEDER: PTDC/CTM-TEX/28295/2017
dc.description.sponsorshipIdPOCI: PTDC/CTM-TEX/28295/2017
dc.format.extent14
dc.identifierhttp://dx.doi.org/10.3389/fbioe.2019.00421
dc.identifier.citationFrontiers In Bioengineering And Biotechnology. Lausanne: Frontiers Media Sa, v. 7, 14 p., 2019.
dc.identifier.doi10.3389/fbioe.2019.00421
dc.identifier.issn2296-4185
dc.identifier.urihttp://hdl.handle.net/11449/195095
dc.identifier.wosWOS:000505485500001
dc.language.isoeng
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers In Bioengineering And Biotechnology
dc.sourceWeb of Science
dc.subjectPLA
dc.subjectnanocomposites
dc.subjectelectrospinning
dc.subjectcutaneous scarring
dc.subjectgene expression
dc.subjectin vivo
dc.titleElectrospun Nanofibrous Poly (Lactic Acid)/Titanium Dioxide Nanocomposite Membranes for Cutaneous Scar Minimizationen
dc.typeArtigo
dcterms.rightsHolderFrontiers Media Sa
unesp.author.orcid0000-0001-5505-2316[5]
unesp.author.orcid0000-0001-5299-4164[11]

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