IgG, IgM and IgA antibody response for the diagnosis and follow-up of paracoccidioidomycosis: Comparison of counterimmunoelectrophoresis and complement fixation

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dc.contributor.authorBueno, J. P.
dc.contributor.authorMendes-Giannini, Maria José Soares [UNESP]
dc.contributor.authorDel Negro, G. M. B.
dc.contributor.authorAssis, C. M.
dc.contributor.authorTakiguti, C. K.
dc.contributor.authorShikanai-Yasuda, M. A.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:18:15Z
dc.date.available2014-05-27T11:18:15Z
dc.date.issued1997-07-22
dc.description.abstractIgG, IgM and IgA antibodies to GP43 (glycoprotein fraction of Paracoccidioides brasiliensis) were measured by ELISA in 63 samples from 23 patients with paracoccidioidomycosis before and twice after chemotherapy was started. Antibodies against P. brasiliensis were detected by indirect immunofluorescence (IF) (IgG, IgM and IgA isotypes), counterimmunoelectrophoresis (CIE) and complement fixation. Two control groups composed of 19 healthy individuals and 12 patients with other diseases (six with histoplasmosis, three with tuberculosis and three with other mycoses). The highest efficiency percentages were found with IgG and IgA- ELISA (100%), IgG-IF (96.2%), CIE (94.4%) and the lowest with CF (75.9%). Highest positive and negative predictive values (100%) were observed for IgG and IgA ELISA. IgG and IgM-ELISA antibodies are more often found in patients with acute than chronic disease (P = 0.01). Four to six months after treatment follow-up showed decreased levels of IgG and IgM-ELISA for acute cases and decreased titres of CIE for chronic cases in relation to pretreatment levels. This study suggests that IgG-ELISA anti-GP43 represents a good marker to monitor clinical response to therapy.en
dc.description.affiliationDepto. Doenças Infecciosas e Parasitárias da F. Medicina da USP Lab. Invest. Médica (LIM 48) H. Clínicas, F. Medicina da USP
dc.description.affiliationLaboratório de Micologia Médico (LIM 53) H. Clínicas, F. Medicina da USP
dc.description.affiliationLab. Processamento de Dados Biomédicos (LIM 39) H. Clínicas, F. Medicina da USP
dc.description.affiliationF. Ciêndas Farmacêuticas UNESP
dc.description.affiliationDepartamento de Doenças Infecciosas e Parasitárias F. Medicina USP LIM 48, Av. Dr Enéas Carvalho Aguiar, CEP 05403 902, São Paulo
dc.description.affiliationUnespF. Ciêndas Farmacêuticas UNESP
dc.format.extent213-217
dc.identifierhttp://dx.doi.org/10.1080/02681219780001161
dc.identifier.citationJournal of Medical and Veterinary Mycology, v. 35, n. 3, p. 213-217, 1997.
dc.identifier.doi10.1080/02681219780001161
dc.identifier.issn0268-1218
dc.identifier.issn1369-3786
dc.identifier.issn1460-2709
dc.identifier.orcid0000-0002-8059-0826
dc.identifier.scopus2-s2.0-0030836629
dc.identifier.scopus2-s2.0-79961230907
dc.identifier.urihttp://hdl.handle.net/11449/65151
dc.language.isoeng
dc.relation.ispartofJournal of Medical and Veterinary Mycology
dc.relation.ispartofjcr2.799
dc.relation.ispartofsjr0,973
dc.relation.ispartofsjr0,973
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectDiagnosis
dc.subjectELISA anti-GP43
dc.subjectP. brasiliensis
dc.subjectParacoccidioidomycosis
dc.subjectglycoprotein
dc.subjectimmunoglobulin a antibody
dc.subjectimmunoglobulin g antibody
dc.subjectimmunoglobulin m antibody
dc.subjectantibody response
dc.subjectbiopsy
dc.subjectcomplement fixation
dc.subjectcontrolled study
dc.subjectcounter immunoelectrophoresis
dc.subjectenzyme linked immunosorbent assay
dc.subjectfollow up
dc.subjectfungus identification
dc.subjecthistoplasma capsulatum
dc.subjecthuman
dc.subjectimmunofluorescence
dc.subjectmajor clinical study
dc.subjectparacoccidioides brasiliensis
dc.subjectsouth american blastomycosis
dc.subjectsputum analysis
dc.subjectAcute Disease
dc.subjectAntibodies, Fungal
dc.subjectAntibody Formation
dc.subjectAntifungal Agents
dc.subjectChronic Disease
dc.subjectComplement Fixation Tests
dc.subjectCounterimmunoelectrophoresis
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectFluorescent Antibody Technique, Indirect
dc.subjectHumans
dc.subjectImmunoglobulin A
dc.subjectImmunoglobulin G
dc.subjectImmunoglobulin M
dc.subjectReference Values
dc.subjectAjellomyces capsulatus
dc.subjectFungi
dc.subjectParacoccidioides brasiliensis
dc.subjectparacoccidioidomycosis brasiliensis
dc.titleIgG, IgM and IgA antibody response for the diagnosis and follow-up of paracoccidioidomycosis: Comparison of counterimmunoelectrophoresis and complement fixationen
dc.typeArtigo
dcterms.licensehttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
unesp.author.orcid0000-0002-8059-0826[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentAnálises Clínicas - FCFpt

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