The influence of pre-treatment on germination of three species of Catasetum (Orchidaceae)

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dc.contributor.authorMoraes, Milena Cristina
dc.contributor.authorBertão, Monica Rosa [UNESP]
dc.contributor.authorCustódio, Ceci Castilho
dc.contributor.authorMachado-Neto, Nelson Barbosa
dc.contributor.institutionAgronomy College
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T16:13:17Z
dc.date.available2023-07-29T16:13:17Z
dc.date.issued2023-01-01
dc.description.abstractAsymbiotic germination techniques are successful in species of the Catasetum genus. The diverse composition of the culture media can be critical; however, viable seeds are required for asymbiotic germination. The reliable method for viability assessment is the tetrazolium test. A decisive step in the efficiency of the tetrazolium test is the seeds preconditioning with sucrose solution, which has the potential to improve germination. Therefore, this work aimed to evaluate the seed germination of three species of the genus Catasetum in culture media, preconditioned or not with sucrose solution. Seeds of the species Catasetum osculatum, Ctsm. galeritum and Ctsm. complanatum were assessed by tetrazolium test and germinated in two different media, pretreated or not with 10% sucrose solution. The statistical analysis showed that the use of sucrose pretreatment significantly increased the germination rates of the species. We conclude that the pretreatment with 10% sucrose for 24 hours, regardless of the species and the culture medium, significantly increases the germination of Ctsm. complanatum, Ctsm. galeritum and Ctsm. osculatum seeds.en
dc.description.affiliationUnoeste Campus II Agronomy College, Rod. Raposo Tavares Km 572, Bairro Limoeiro, SP
dc.description.affiliationUniversidade Estadual Paulista (Unesp) School of Sciences Humanities and Languages Department of Biotechnology, Avenida Dom Antonio, 2100, SP
dc.description.affiliationUnespUniversidade Estadual Paulista (Unesp) School of Sciences Humanities and Languages Department of Biotechnology, Avenida Dom Antonio, 2100, SP
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdCAPES: 001
dc.identifierhttp://dx.doi.org/10.1590/2317-1545v45266264
dc.identifier.citationJournal of Seed Science, v. 45.
dc.identifier.doi10.1590/2317-1545v45266264
dc.identifier.issn2317-1537
dc.identifier.scopus2-s2.0-85158895860
dc.identifier.urihttp://hdl.handle.net/11449/249938
dc.language.isoeng
dc.relation.ispartofJournal of Seed Science
dc.sourceScopus
dc.subjectorchid
dc.subjectplant culture
dc.subjectseeds
dc.subjectsucrose solution
dc.subjecttetrazolium
dc.titleThe influence of pre-treatment on germination of three species of Catasetum (Orchidaceae)en
dc.typeArtigo
unesp.departmentCiências Biológicas - FCLASpt

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