Mutagenic, genotoxic and morphotoxic potential of different pesticides in the erythrocytes of Podocnemis expansa neonates

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dc.contributor.authorPereira de Oliveira, Jose Silonardo
dc.contributor.authorVieira, Lucelia Goncalves
dc.contributor.authorCarvalho, Wanessa Fernandes
dc.contributor.authorSouza, Marcelino Benvindo de
dc.contributor.authorLima Rodrigues, Aline Sueli de
dc.contributor.authorSimoes, Karina
dc.contributor.authorDe Silva, Daniela de Melo
dc.contributor.authorMendonca, Juliana dos Santos [UNESP]
dc.contributor.authorLuz Hirano, Liria Queiroz
dc.contributor.authorQuagliatto Santos, Andre Luiz
dc.contributor.authorMalafaia, Guilherme
dc.contributor.institutionGoiano Fed Inst Urutai
dc.contributor.institutionFed Univ Goiania
dc.contributor.institutionICB I Fed Univ Goias
dc.contributor.institutionUniversidade Federal de Goiás (UFG)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de Brasília (UnB)
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.date.accessioned2020-12-10T17:38:30Z
dc.date.available2020-12-10T17:38:30Z
dc.date.issued2020-10-01
dc.description.abstractDespite the damaging effects of pesticides glyphosate (Gly), atrazine (Atra) and fipronil (lip) on different organisms, the mutagenic, genotoxic and morphotoxic potential of testudine erythrocytes in freshwater remains unknown. Thus, the aim of the present study is to assess the toxicological potential of these compounds in Podocnemis expansa (Amazonian turtles) neonates from eggs artificially incubated in substrate at different concentrations of herbicides Gly and Atra and insecticide Pip. Micronucleus test and other nuclear abnormalities, as well as comet assay and morphometric measurements taken of models circulating erythrocytes were used as toxicity biomarkers. Pups exposed to Gly (groups Gly-65 ppb and Gly-6500 ppb) were the ones recording the largest amount of nuclear abnormalities; erythrocytes with multilobulated, notched and displaced nucleus were mostly frequent in groups Atra-2 ppb and Gly-65 ppb. All treatments (Gly-6500 ppb, Atra-2 ppb, Atra-200 ppb, Fip-4 ppb and Fip-400 ppb), except for group Gly-65 ppb, led to decreased erythrocyte area, increased nuclear area: erythrocyte area ratio, as well as to decreased erythrocyte and erythrocyte nuclei circularity, which highlights the clear effect on the size and shape of these cells. On the other hand, the cornet assay did not evidence any genotoxic effect caused by the assessed pesticides. This is a pioneer study on the mutagenic and morphotoxic potential of pesticides in P. expansa eclodides exposed in ovo to Gly, Atra and Fip; therefore, it is an insight on how these compounds can affect the health of these animals. (C) 2020 Elsevier B.V. All rights reserved.en
dc.description.affiliationGoiano Fed Inst Urutai, Biol Res Lab, Postgrad Program Conservat Cerrado Nat Resources, Urutai, Go, Brazil
dc.description.affiliationFed Univ Goiania, Biol Sci Inst, Goiania, Go, Brazil
dc.description.affiliationICB I Fed Univ Goias, Biol Sci Inst, Mutagenesis Lab, Samambaia Campus, Goiania, Go, Brazil
dc.description.affiliationUniv Fed Goias, Biol Sci Inst, Morphol Dept, Samambaia Campus, Goiania, Go, Brazil
dc.description.affiliationPaulista State Univ Julio de Mesquita Filho UNESP, Inst Biosci, Rio Claro, SP, Brazil
dc.description.affiliationUniv Brasilia, Brasiliae, DF, Brazil
dc.description.affiliationUniv Fed Uberlandia, Wild Anim Teaching & Res Lab, Uberlandia, MG, Brazil
dc.description.affiliationUnespPaulista State Univ Julio de Mesquita Filho UNESP, Inst Biosci, Rio Claro, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipInstituto Federal Goiano
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFAPEG
dc.description.sponsorshipNational Center for Research and Conservation of Reptiles and Amphibians (RAN) of the Chico Mendes Institute for Biodiversity Conservation (ICMBio)
dc.description.sponsorshipIdCNPq: 426531/2018-3
dc.description.sponsorshipIdInstituto Federal Goiano: 23219.001925.2019-36
dc.description.sponsorshipIdCNPq: 307743/2018-7
dc.format.extent9
dc.identifierhttp://dx.doi.org/10.1016/j.scitotenv.2020.140304
dc.identifier.citationScience Of The Total Environment. Amsterdam: Elsevier, v. 737, 9 p., 2020.
dc.identifier.doi10.1016/j.scitotenv.2020.140304
dc.identifier.issn0048-9697
dc.identifier.urihttp://hdl.handle.net/11449/195553
dc.identifier.wosWOS:000553719700016
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofScience Of The Total Environment
dc.sourceWeb of Science
dc.subjectPesticides
dc.subjectEcotoxicology
dc.subjectMutagenicity
dc.subjectGenotoxicity
dc.subjectMorphotoxicity
dc.titleMutagenic, genotoxic and morphotoxic potential of different pesticides in the erythrocytes of Podocnemis expansa neonatesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0003-1818-4111[5]
unesp.author.orcid0000-0003-4773-7598[11]

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