Comparison of superovulatory responses to a standardized hormonal superstimulation protocol among three indigenous breeds of sheep in Brazil

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dc.contributor.authorPupin, Maria Amélia Ferrão [UNESP]
dc.contributor.authorOliveira, Maria Emilia Franco [UNESP]
dc.contributor.authorVergani, Gabriel Brun [UNESP]
dc.contributor.authorLima, Monalisa Sousa Dias
dc.contributor.authorSilva, Kleibe de Moraes
dc.contributor.authorMonteiro, Alexandre Weick Uchôa
dc.contributor.authorRamos, Alexandre Floriani
dc.contributor.authorBatista, Ribrio Ivan Tavares Pereira
dc.contributor.authorSouza-Fabjan, Joanna Maria Gonçalves
dc.contributor.authorBartlewski, Pawel Mieczyslaw
dc.contributor.authorFonseca, Jeferson Ferreira
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionUniversidade Estadual do Ceará
dc.contributor.institutionUniversidade Federal do Vales dos Jequitinhonha e Mucuri
dc.contributor.institutionUniversidade Federal Fluminense (UFF)
dc.contributor.institutionUniversity of Guelph
dc.date.accessioned2023-03-01T19:57:04Z
dc.date.available2023-03-01T19:57:04Z
dc.date.issued2022-06-01
dc.description.abstractThis study examined the differences in ovarian responses and in vivo embryo production in three naturalized Brazilian sheep breeds (Morada Nova, Santa Inês, and Somalis Brasileira). Thirty cycling, multiparous ewes (n = 10/breed) received intravaginal devices containing 0.33 g of progesterone (CIDR®) for 9 days and the twice-daily superovulatory treatment with 133 mg of porcine follicle-stimulating hormone (six decreasing doses) started on Day 7 (Day 0 PM = CIDR® insertion). On Days 11 (36 h after CIDR® removal) and 15 (12 h before non-surgical embryo recovery performed seven days after CIDR® removal), all ewes were examined using B-mode and Doppler ultrasonography. The number of medium-sized ovarian follicles (4.0–5.99 mm) on Day 11 was greater (P = 0.003) in Santa Inês and Somalis Brasileira compared with Morada Nova ewes. Small antral follicle numbers (2.0–3.99 mm in diameter) on Day 11 were directly related to ovulatory responses in all three breeds of superovulated ewes. The number of corpora lutea on Day 15 was greater (P < 0.0001) in Santa Inês, followed by Somalis Brasileira and Morada Nova ewes; however, the number of viable embryos and viability rate did not differ (P > 0.05) among the three genotypes of ewes studied. The total ovarian area (TA), color Doppler area (DA), and DA/TA × 100% increased from Day 11 to Day 15 in all breeds, and they were lowest (P < 0.001) in superovulated Morada Nova sheep. Significant positive correlations among ovarian antral follicle numbers in different size classes (Day 11) and ovulatory responses were recorded in all breeds, but associations between the ovarian blood perfusion and superovulatory outcomes were restricted to the Morada Nova and Santa Inês genotypes. In summary, ovarian follicle numbers and blood flow indices differed among the three naturalized Brazilian ewes, but no significant differences were noted in embryo yields and quality following superovulation. Small antral follicle count 36 h after CIDR® treatment was a reliable predictor of impending ovulation rates in all ewes under study.en
dc.description.affiliationFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista, SP
dc.description.affiliationEmbrapa Caprinos e Ovinos, CE
dc.description.affiliationUniversidade Estadual do Ceará, CE
dc.description.affiliationEmbrapa Recursos Genéticos e Biotecnologia, DF
dc.description.affiliationUniversidade Federal do Vales dos Jequitinhonha e Mucuri, MG
dc.description.affiliationUniversidade Federal Fluminense, RJ
dc.description.affiliationDepartment of Biomedical Sciences Ontario Veterinary College University of Guelph
dc.description.affiliationUnespFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista, SP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipIdFAPEMIG: 00201-17
dc.identifierhttp://dx.doi.org/10.1016/j.smallrumres.2022.106703
dc.identifier.citationSmall Ruminant Research, v. 211.
dc.identifier.doi10.1016/j.smallrumres.2022.106703
dc.identifier.issn0921-4488
dc.identifier.scopus2-s2.0-85129484576
dc.identifier.urihttp://hdl.handle.net/11449/240001
dc.language.isoeng
dc.relation.ispartofSmall Ruminant Research
dc.sourceScopus
dc.subjectColor Doppler
dc.subjectCorpora lutea
dc.subjectEwe
dc.subjectOvarian antral follicles
dc.subjectOvarian blood perfusion
dc.subjectSuperovulation
dc.titleComparison of superovulatory responses to a standardized hormonal superstimulation protocol among three indigenous breeds of sheep in Brazilen
dc.typeArtigo

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