Effects of long-term fluoride exposure are associated with oxidative biochemistry impairment and global proteomic modulation, but not genotoxicity, in parotid glands of mice

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dc.contributor.authorMiranda, Giza Hellen Nonato
dc.contributor.authorDe Oliveira Lima, Leidiane Alencar
dc.contributor.authorBittencourt, Leonardo Oliveira
dc.contributor.authorDos Santos, Savio Monteiro
dc.contributor.authorDe Souza, Michel Platini Caldas
dc.contributor.authorNogueira, Lygia Sega
dc.contributor.authorDe Oliveira, Edivaldo Herculano Corrêa
dc.contributor.authorMonteiro, Marta Chagas
dc.contributor.authorDionizio, Aline
dc.contributor.authorLeite, Aline Lima
dc.contributor.authorPessan, Juliano Pelim [UNESP]
dc.contributor.authorBuzalaf, Marilia Afonso Rabelo
dc.contributor.authorLima, Rafael Rodrigues
dc.contributor.institutionFederal University of Para
dc.contributor.institutionEvandro Chagas Institute
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversity of Nebraska-Lincoln
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T08:46:34Z
dc.date.available2022-04-29T08:46:34Z
dc.date.issued2022-01-01
dc.description.abstractBackground Fluoride has become widely used in dentistry because of its effectiveness in caries control. However, evidence indicates that excessive intake interferes with the metabolic processes of different tissues. Thus, this study aimed to investigate the effects of long-term exposure to F on the parotid salivary gland of mice, from the analysis of oxidative, proteomic and genotoxic parameters. Materials and methods The animals received deionized water containing 0, 10 or 50 mg/L of F, as sodium fluoride, for 60 days. After, parotid glands were collected for analysis of oxidative biochemistry, global proteomic profile, genotoxicity assessment and histopathological analyses. Results The results revealed that exposure to fluoride interfered in the biochemical homeostasis of the parotid gland, with increased levels of thiobarbituric acid reactive species and reduced glutathione in the exposed groups; as well as promoted alteration of the glandular proteomic profile in these groups, especially in structural proteins and proteins related to oxidative stress. However, genotoxic assessment demonstrated that exposure to fluoride did not interfere with DNA integrity in these concentrations and durations of exposure. Also, it was not observed histopathological alterations in parotid gland. Conclusions Thus, our results suggest that long-term exposure to fluoride promoted modulation of the proteomic and biochemical profile in the parotid glands, without inducing damage to the genetic component. These findings reinforce the importance of rationalizing the use of fluorides to maximize their preventative effects while minimizing the environmental risks.en
dc.description.affiliationLaboratory of Functional and Structural Biology Institute of Biological Sciences Federal University of Para, PA
dc.description.affiliationLaboratory of Clinical Immunology and Oxidative Stress Pharmacy Faculty Institute of Health Science Federal University of Para, PA
dc.description.affiliationEvandro Chagas Institute, PA
dc.description.affiliationDepartment of Biological Sciences Bauru Dental School University of São Paulo, SP
dc.description.affiliationDepartment of Chemistry University of Nebraska-Lincoln
dc.description.affiliationDepartment of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP), SP
dc.description.affiliationUnespDepartment of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP), SP
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0261252
dc.identifier.citationPLoS ONE, v. 17, n. 1 January, 2022.
dc.identifier.doi10.1371/journal.pone.0261252
dc.identifier.issn1932-6203
dc.identifier.scopus2-s2.0-85123743047
dc.identifier.urihttp://hdl.handle.net/11449/231610
dc.language.isoeng
dc.relation.ispartofPLoS ONE
dc.sourceScopus
dc.titleEffects of long-term fluoride exposure are associated with oxidative biochemistry impairment and global proteomic modulation, but not genotoxicity, in parotid glands of miceen
dc.typeArtigo
unesp.departmentCiências Biológicas - FCpt

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