Growth-promoting factors for yeast cells of Paracoccidioides brasiliensis

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dc.contributor.authorKurita, Nobuyuki
dc.contributor.authorSano, Ayako
dc.contributor.authorSoares, Angela
dc.contributor.authorMendes, Rinaldo P.
dc.contributor.authorFranco, Marcello F.
dc.contributor.authorNishimura, Kazuko
dc.contributor.authorMiyaji, Makoto
dc.contributor.institutionChiba University
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:18:08Z
dc.date.available2014-05-27T11:18:08Z
dc.date.issued1996-12-01
dc.description.abstractLow-density seedings of yeast cells of Paracoccidioides brasiliensis give poor growth (as assessed by plating efficiency test) on conventional mycological agar media, and therefore growth-promoting factors for this fungus were sought. Water-extracts of yeast cells of six P. brasiliensis isolates were all considerably effective in promoting the growth of low-density seedings of P. brasiliensis isolates Pb-18 and Hachisuga, but had little effect on isolate Bt-4. Horse serum, at a concentration range of 2-4%, moderately or considerably promoted the growth of these P. brasiliensis isolates. Combinations of the fungus cell extracts with horse serum were highly effective in promoting the growth of all of the fungal isolates. The fungus cell extracts showed siderophore (microbial iron carrier) activity. An iron-chelator, ethylenediaminetetraacetic acid, at a concentration of 100 μM also highly promoted the growth of the fungal isolates in the presence of horse serum, and ferric ion added to culture medium was considerably effective in the growth promotion. These results suggest that deficient utilization of external iron by the fungus cell is one of the growth-limiting processes for low-density seedings of yeast cells of P. brasiliensis on conventional mycological agar media.en
dc.description.affiliationRes. Ctr. Pathogenic Fungi/M.T. Chiba University, 1-8-1, Inohana, Chuo-ku, Chiba-shi, Chiba 260
dc.description.affiliationInstitute of Biosciences Univ. of the State of S. Paulo, 18618-000 Botucatu, Sao Paulo
dc.description.affiliationBotucatú Medical School Univ. of the State of S. Paulo, 18618-000 Botucatu, Sao Paulo
dc.format.extent35-39
dc.identifierhttp://dx.doi.org/10.1007/BF02461454
dc.identifier.citationMycoscience, v. 37, n. 1, p. 35-39, 1996.
dc.identifier.doi10.1007/BF02461454
dc.identifier.issn1340-3540
dc.identifier.scopus2-s2.0-0343982530
dc.identifier.urihttp://hdl.handle.net/11449/64891
dc.language.isoeng
dc.relation.ispartofMycoscience
dc.relation.ispartofjcr1.229
dc.relation.ispartofsjr0,804
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectIron
dc.subjectIron chelator
dc.subjectParacoccidioides brasiliensis
dc.subjectPlating efficiency
dc.subjectSiderophore
dc.subjectEquus caballus
dc.subjectFungi
dc.titleGrowth-promoting factors for yeast cells of Paracoccidioides brasiliensisen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy

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