Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina

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dc.contributor.authorFusco, Luciano S.
dc.contributor.authorNeto, Emidio B.
dc.contributor.authorFrancisco, Aleff F. [UNESP]
dc.contributor.authorAlfonso, Jorge
dc.contributor.authorSoares, Andreimar
dc.contributor.authorPimenta, Daniel C.
dc.contributor.authorLeiva, Laura C.
dc.contributor.institutionUniversidad Nacional del Nordeste (UNNE)
dc.contributor.institutionInstituto Butantan
dc.contributor.institutionPorto Velho-RO e Universidade Federal de Rondônia (UNIR)
dc.contributor.institutionCentro Para el Desarrollo de la Investigación Científica (CEDIC)
dc.contributor.institutionCentro Universitário São Lucas (UniSL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-12T01:28:08Z
dc.date.available2020-12-12T01:28:08Z
dc.date.issued2020-09-01
dc.description.abstractThe complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in the C.d. terrificus venom, a Southern American Neotropical rattlesnake. The RP-HPLC separation profile of pooled venom from adult specimens followed by mass spectrometry analysis revealed that C.d. terrificus’ venom proteome is composed of 12 protein families, which are unevenly distributed in the venom, e.g., there are few major proteins in the venom's composition phospholipase A2, serine proteinase, crotamine and L-amino acid oxidase. At the same time, the proteome analysis revealed a small set of proteins with low quantity (less than 1.5%), both enzymes (metaloprotease, phospholipase B and 5′-nucleotidase) and proteins (Bradykinin potentiating and C-type natriuretic peptides, C-type lectin convulxin and nerve growth factor). To sum up, this research is the first venomic report of C.d.terrificus venom from Argentina. This proved to be crotamine positive venom that has a lower metalloprotease content than C.d. terrificus venoms from other regions. This information could be used in the discovery of future pharmacological agents or targets in antivenom therapy.en
dc.description.affiliationLaboratorio de Investigación en Proteínas (LabInPro) Instituto de Química Básica y aplicada del Nordeste Argentino (IQUIBA-NEA CONICET) Universidad Nacional del Nordeste (UNNE)
dc.description.affiliationLaboratorio de Bioquímica y Biofísica Instituto Butantan
dc.description.affiliationCentro de Estudos de Biomoléculas Aplicadas à Saúde (CEBio) Fundação Oswaldo Cruz (FIOCRUZ) Fiocruz Rondônia Porto Velho-RO e Universidade Federal de Rondônia (UNIR)
dc.description.affiliationCentro Para el Desarrollo de la Investigación Científica (CEDIC)
dc.description.affiliationCentro Universitário São Lucas (UniSL)
dc.description.affiliationDepartamento de Física e Biofísica Instituto de Biociências São Paulo State University UNESP
dc.description.affiliationUnespDepartamento de Física e Biofísica Instituto de Biociências São Paulo State University UNESP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas
dc.description.sponsorshipConsejo Nacional de Ciencia y Tecnología, Paraguay
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipUniversidad Nacional del Nordeste
dc.description.sponsorshipFinanciadora de Estudos e Projetos
dc.description.sponsorshipFondo para la Investigación Científica y Tecnológica
dc.description.sponsorshipIdCAPES: #001
dc.description.sponsorshipIdFinanciadora de Estudos e Projetos: #01.09.0278.04
dc.description.sponsorshipIdFinanciadora de Estudos e Projetos: #01.12.0450.03
dc.description.sponsorshipIdCNPq: #303792/2016-7
dc.description.sponsorshipIdCNPq: 406385/2018-1
dc.description.sponsorshipIdFondo para la Investigación Científica y Tecnológica: PICT-JOVEN 038
dc.identifierhttp://dx.doi.org/10.1016/j.toxcx.2020.100047
dc.identifier.citationToxicon: X, v. 7.
dc.identifier.doi10.1016/j.toxcx.2020.100047
dc.identifier.issn2590-1710
dc.identifier.scopus2-s2.0-85086652377
dc.identifier.urihttp://hdl.handle.net/11449/199004
dc.language.isoeng
dc.relation.ispartofToxicon: X
dc.sourceScopus
dc.titleFast venomic analysis of Crotalus durissus terrificus from northeastern Argentinaen
dc.typeArtigo

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