Prenatal ethanol exposure affects the proliferation and differentiation of the osteoblasts from newborn rats
Carregando...
Data
2015-01-01
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Tipo
Artigo
Direito de acesso
Acesso aberto![Acesso Aberto](assets/repositorio/images/logo_acesso_aberto_simples.png)
![Acesso Aberto](assets/repositorio/images/logo_acesso_aberto_simples.png)
Resumo
Alcohol exerts teratogenic effects and its consumption during pregnancy may cause various alterations in the fetus, including deficit of bone development. The objective of this study was to evaluate the initial responses, on osteoblasts isolated from newborn rat calvaria, after prenatal ethanol exposure. Nine pregnant rats were divided into three groups according to the diet fed during pregnancy: Rats fed 20% ethanol, Pair-fed and control were the groups. At 3 days of life, newborn rats were euthanized for removal of the calvaria and isolation of osteogenic cells by sequential enzymatic digestion. The cells were cultured for a maximum period of 14 days. The effect of alcohol was investigated by the measurement of cell adhesion, proliferation and viability, total protein content, Alkaline Phosphatase (ALP) activity and bone matrix formation. The results showed the highest proliferation in ETH group on the 3<sup>th</sup> day and the highest ALP activity and bone matrix formation, in this group, on the 14<sup>th</sup> day, indicating that prenatal ethanol seems to affect the proliferation earlyand the ALP activity and bone matrix formation in more advanced periods.
Descrição
Palavras-chave
Idioma
Inglês
Como citar
OnLine Journal of Biological Sciences, v. 15, n. 3, p. 134-142, 2015.