Prenatal ethanol exposure affects the proliferation and differentiation of the osteoblasts from newborn rats

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dc.contributor.authorCarvalho, Isabel Chaves Silva [UNESP]
dc.contributor.authorDe Andrade, Dennia Perez [UNESP]
dc.contributor.authorMilhan, Noala Vicensoto Moreira [UNESP]
dc.contributor.authorDe Souza Santos, Evelyn Luzia [UNESP]
dc.contributor.authorSoares, Cristina Pacheco
dc.contributor.authorDa Rocha, Rosilene Fernandes [UNESP]
dc.contributor.authorDe Vasconcellos, Luana Marotta Reis [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInstitute of Research and Development-IPandD, Universidade do Vale do Paraíba-UNIVAP, Laboratory Dynamics of Cellular Compartments
dc.date.accessioned2018-12-11T16:58:11Z
dc.date.available2018-12-11T16:58:11Z
dc.date.issued2015-01-01
dc.description.abstractAlcohol exerts teratogenic effects and its consumption during pregnancy may cause various alterations in the fetus, including deficit of bone development. The objective of this study was to evaluate the initial responses, on osteoblasts isolated from newborn rat calvaria, after prenatal ethanol exposure. Nine pregnant rats were divided into three groups according to the diet fed during pregnancy: Rats fed 20% ethanol, Pair-fed and control were the groups. At 3 days of life, newborn rats were euthanized for removal of the calvaria and isolation of osteogenic cells by sequential enzymatic digestion. The cells were cultured for a maximum period of 14 days. The effect of alcohol was investigated by the measurement of cell adhesion, proliferation and viability, total protein content, Alkaline Phosphatase (ALP) activity and bone matrix formation. The results showed the highest proliferation in ETH group on the 3<sup>th</sup> day and the highest ALP activity and bone matrix formation, in this group, on the 14<sup>th</sup> day, indicating that prenatal ethanol seems to affect the proliferation earlyand the ALP activity and bone matrix formation in more advanced periods.en
dc.description.affiliationInstitute of Science and Technology, UNESP-Univ Estadual Paulista, Sao Jose dos Campos (SP), School of Dentistry, Department of Biosciences and Oral Diagnosis
dc.description.affiliationInstitute of Research and Development-IPandD, Universidade do Vale do Paraíba-UNIVAP, Laboratory Dynamics of Cellular Compartments
dc.description.affiliationUnespInstitute of Science and Technology, UNESP-Univ Estadual Paulista, Sao Jose dos Campos (SP), School of Dentistry, Department of Biosciences and Oral Diagnosis
dc.format.extent134-142
dc.identifierhttp://dx.doi.org/10.3844/ojbsci.2015.134.142
dc.identifier.citationOnLine Journal of Biological Sciences, v. 15, n. 3, p. 134-142, 2015.
dc.identifier.doi10.3844/ojbsci.2015.134.142
dc.identifier.file2-s2.0-84940176419.pdf
dc.identifier.issn1608-4217
dc.identifier.scopus2-s2.0-84940176419
dc.identifier.urihttp://hdl.handle.net/11449/172024
dc.language.isoeng
dc.relation.ispartofOnLine Journal of Biological Sciences
dc.relation.ispartofsjr0,166
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCell culture
dc.subjectEthanol
dc.subjectGestation
dc.subjectOsteoblasts
dc.subjectRat
dc.titlePrenatal ethanol exposure affects the proliferation and differentiation of the osteoblasts from newborn ratsen
dc.typeArtigo

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