Which Histometric Analysis Approach Is More Reliable for Assessing Histological Bone Tissue Samples?

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dc.contributor.authorPereira, Rodrigo dos Santos
dc.contributor.authorMourão, Carlos Fernando
dc.contributor.authorPiattelli, Adriano
dc.contributor.authorRomanos, Georgios E.
dc.contributor.authorCoelho Mendes, Bruno [UNESP]
dc.contributor.authorGiubilato, Flavio [UNESP]
dc.contributor.authorMontemezzi, Pietro
dc.contributor.authorJúnior Conforte, Jadson [UNESP]
dc.contributor.authorGriza, Geraldo Luiz [UNESP]
dc.contributor.authorBonardi, João Paulo [UNESP]
dc.contributor.authorHochuli-Vieira, Eduardo [UNESP]
dc.contributor.institutionUniversity of Grande Rio-UNIGRANRIO
dc.contributor.institutionTufts University School of Dental Medicine
dc.contributor.institutionUniversity “G. D’Annunzio” of Chieti-Pescara
dc.contributor.institutionStony Brook University
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionFederal Fluminense University
dc.contributor.institutionSan Raffaele Hospital
dc.date.accessioned2023-07-29T13:25:58Z
dc.date.available2023-07-29T13:25:58Z
dc.date.issued2022-10-01
dc.description.abstractThis study aims to evaluate the grid of Merz and ImageJ methods for histometric quantification, verifying which is more reliable and defining which is most suitable based on the time required to perform. Thirty histological samples of maxillary sinuses grafted with xenografts were evaluated using an optical light microscope attached to an image capture camera and connected to a microcomputer. The images were digitalized and recorded as a TIFF image, and the new bone formation was evaluated using the grid of Merz and ImageJ. The Bland–Altman analysis was used to identify the agreement between the methods and determine suitable future research options. The timing of the quantification was also performed to identify a possible advantage. The mean value for the quantification analysis timing for the grid of Merz was 194.9 ± 72.0 s and for ImageJ was 871.7 ± 264.4, with statistical significance between the groups (p = 0.0001). The Bland–Altman analysis demonstrated a concordance between the methods, due to the bias being next to the maximum concordance (−1.25) in addition to the graphic showing the scattering points next to the mean of differences and inside of limits of agreement. Thus, it was demonstrated that the grid of Merz presents reliable outcomes and advantages over the ImageJ methodology regarding the time spent to contour the areas of interest.en
dc.description.affiliationDepartment of Oral Maxillofacial Surgery University of Grande Rio-UNIGRANRIO
dc.description.affiliationDepartment of Periodontology Dental Research Administration Tufts University School of Dental Medicine
dc.description.affiliationDepartment of Medical Oral and Biotechnological Sciences University “G. D’Annunzio” of Chieti-Pescara
dc.description.affiliationSchool of Dental Medicine Stony Brook University
dc.description.affiliationDepartment of Oral Maxillofacial Surgery Aracatuba School of Dentistry Sao Paulo State University
dc.description.affiliationClinical Research Laboratory in Dentistry Federal Fluminense University
dc.description.affiliationDepartment of Dentistry San Raffaele Hospital
dc.description.affiliationDepartment of Diagnostic and Surgery Aracatuba School of Dentistry Sao Paulo State University
dc.description.affiliationUnespDepartment of Oral Maxillofacial Surgery Aracatuba School of Dentistry Sao Paulo State University
dc.description.affiliationUnespDepartment of Diagnostic and Surgery Aracatuba School of Dentistry Sao Paulo State University
dc.identifierhttp://dx.doi.org/10.3390/medicina58101364
dc.identifier.citationMedicina (Lithuania), v. 58, n. 10, 2022.
dc.identifier.doi10.3390/medicina58101364
dc.identifier.issn1648-9144
dc.identifier.issn1010-660X
dc.identifier.scopus2-s2.0-85140586173
dc.identifier.urihttp://hdl.handle.net/11449/247793
dc.language.isoeng
dc.relation.ispartofMedicina (Lithuania)
dc.sourceScopus
dc.subjectBland–Altman analysis
dc.subjecthistological measurement
dc.subjecthistomorphometric analysis
dc.titleWhich Histometric Analysis Approach Is More Reliable for Assessing Histological Bone Tissue Samples?en
dc.typeArtigo
unesp.author.orcid0000-0003-2509-8633[1]
unesp.author.orcid0000-0001-5775-0222[2]
unesp.author.orcid0000-0002-7745-5957[4]

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