The Anti-Inflammatory Effects of the Methanolic Extract and Fractions from Davilla elliptica St. Hil. (Dilleniaceae) on Bothrops jararaca Envenomation

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dc.contributor.authorNishijima, Catarine Massucato [UNESP]
dc.contributor.authorDelella, Flavia Karina [UNESP]
dc.contributor.authorRodrigues, Clenilson Martins
dc.contributor.authorRinaldo, Daniel [UNESP]
dc.contributor.authorAnjos Lopes-Ferreira, Monica Valdyrce dos
dc.contributor.authorMachado da Rocha, Lucia Regina [UNESP]
dc.contributor.authorVilegas, Wagner [UNESP]
dc.contributor.authorFelisbino, Sergio Luis [UNESP]
dc.contributor.authorHiruma-Lima, Clelia Akiko [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionEmpresa Brasileira Pesquisa Agr
dc.contributor.institutionInst Butantan
dc.date.accessioned2015-10-21T21:21:34Z
dc.date.available2015-10-21T21:21:34Z
dc.date.issued2015-06-01
dc.description.abstractInflammation and haemorrhage are the main characteristics of tissue injury in botropic envenomation. Although some studies have shown that anti-venom prevents systemic reactions, it is not efficient in preventing tissue injury at the site of the bite. Therefore, this work was undertaken to investigate the anti-inflammatory effects of the methanolic extract and fractions from D. elliptica and to evaluate the role of matrix metalloproteinases (MMPs) in this process. Effects of the extract and fractions from D. elliptica were evaluated using a carrageenan-induced paw oedema model in rats, and leukocyte rolling was visualized by intravital. The quantification of MMPs activities (MMP-2 and MMP-9) extracted from the dermis of mice treated with extract and fractions alone or incubated with venom was determined by zymographic analyses. Our results show that intraperitoneal (i.p.) injection of fractions significantly reduced paw oedema after the carrageenan challenge. Treatment with the tannins fraction also resulted in considerable inhibition of the rolling of leukocytes and this fraction was able to decrease the activation of MMP-9. These results confirmed the anti-inflammatory activity of the methanolic extract and tannins fraction of D. elliptica and showed that the dermonecrosis properties of B. jararaca venom might be mediated through the inhibition of MMP-9 activity.en
dc.description.affiliationUniv Estadual Paulista UNESP, Inst Biociencias, Dept Fisiol, BR-18618970 Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Inst Biociencias, Dept Morfol, BR-18618970 Sao Paulo, Brazil
dc.description.affiliationEmpresa Brasileira Pesquisa Agr, Ctr Nacl Pesquisa Agroenergia CNPAE, BR-70770901 Brasilia, DF, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Fac Ciencias Bauru, BR-17033360 Bauru, Brazil
dc.description.affiliationInst Butantan, Lab Especial Toxinol Aplicada Butanta, BR-05503900 Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, BR-11330900 Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Inst Biociencias, Dept Fisiol, BR-18618970 Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Inst Biociencias, Dept Morfol, BR-18618970 Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Fac Ciencias Bauru, BR-17033360 Bauru, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, BR-11330900 Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação para o Desenvolvimento da UNESP (FUNDUNESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent12454-12466
dc.identifierhttp://www.mdpi.com/1422-0067/16/6/12454
dc.identifier.citationInternational Journal Of Molecular Sciences, v. 16, n. 6, p. 12454-12466, 2015.
dc.identifier.doi10.3390/ijms160612454
dc.identifier.issn1422-0067
dc.identifier.lattes7927877224326837
dc.identifier.lattes3814504901386844
dc.identifier.orcid0000-0002-8645-3777
dc.identifier.orcid0000-0003-3032-2556
dc.identifier.urihttp://hdl.handle.net/11449/129557
dc.identifier.wosWOS:000357492800038
dc.language.isoeng
dc.publisherMdpi Ag
dc.relation.ispartofInternational Journal Of Molecular Sciences
dc.relation.ispartofjcr3.687
dc.relation.ispartofsjr1,260
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleThe Anti-Inflammatory Effects of the Methanolic Extract and Fractions from Davilla elliptica St. Hil. (Dilleniaceae) on Bothrops jararaca Envenomationen
dc.typeArtigo
dcterms.rightsHolderMdpi Ag
unesp.author.lattes7927877224326837
unesp.author.lattes3814504901386844[9]
unesp.author.orcid0000-0002-8645-3777[9]
unesp.author.orcid0000-0001-5363-6481[4]
unesp.author.orcid0000-0003-3032-2556[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, São Vicentept
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Morfologia - IBB
Artigos - Ciências Biológicas - São Vicente