Optimized electrochemical biosensor for human prostatic acid phosphatase
Carregando...
Fonte externa
Fonte externa
Data
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Tipo
Artigo
Direito de acesso
Acesso aberto
Fonte externa
Fonte externa
Resumo
An innovative and sensitive label-free electrochemical biosensor platform for detecting human prostatic acid phosphatase (hPAP) is proposed herein. The methodology uses impedance-derived immittance functions (ImFs) for monitoring the interfacial changes in a hPAP bio-recognition layer constructed using a self-assembled monolayer of PEGlate thiol (low-fouling materials) and 11-ferrocenyl undecanethiol (redox probe confined). We evaluated the response toward the target primarily in phosphatase-buffered saline, at varying concentrations and across a wide range of frequencies. The optimum frequency for each ImF parameter was selected based on its sensitivity and linearity within a target concentration ranging from 20 pM to 5000 pM and a limit of detection (LoD) below the cut-off value for this cancer biomarker (10 pM). The best ImF selected (inverse of imaginary capacitance, 1/C?) was then successfully used to monitor hPAP in human blood serum and showed a linear range from 50 pM to 104 pM, with an LoD of 11.2 +/- 2.6 pM. Additionally, both the assay and analysis of the results can be performed in a few minutes without the need to add an interfering redox probe to the biological samples, thus providing a rapid, sensitive, and label-free test for prostate cancer. (C) 2017 Elsevier B.V. All rights reserved.
Descrição
Palavras-chave
Immittance spectroscopy, Electrochemical impedance spectroscopy, Prostate cancer, Human prostatic acid phosphatase, Label-free sensing, Electrochemical biosensora
Idioma
Inglês
Citação
Sensors And Actuators B-chemical. Lausanne: Elsevier Science Sa, v. 253, p. 1106-1112, 2017.
