Combined treatment with oestradiol benzoate, d-cloprostenol and oxytocin permits cervical dilation and nonsurgical embryo recovery in ewes

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dc.contributor.authorFonseca, Jeferson Ferreira da
dc.contributor.authorZambrini, Fabiana Nunes
dc.contributor.authorGuimaraes, Jose Domingos
dc.contributor.authorSilva, Marcio Roberto
dc.contributor.authorFranco Oliveira, Maria Emilia [UNESP]
dc.contributor.authorBrandao, Felipe Zandonadi
dc.contributor.authorBartlewski, Pawel Mieczyslaw
dc.contributor.authorGoncalves Souza-Fabjan, Joanna Maria
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionUniversidade Federal de Viçosa (UFV)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal Fluminense (UFF)
dc.contributor.institutionUniv Guelph
dc.date.accessioned2019-10-04T11:56:19Z
dc.date.available2019-10-04T11:56:19Z
dc.date.issued2019-01-01
dc.description.abstractContents This study examined the feasibility of transcervical embryo recovery after the hormonal treatment to induce cervical dilation, following the 7-day oestrous synchronization protocol in multiparous Santa Ines ewes. A total of 23 cyclic ewes received two doses of 37.5 mu g of d-cloprostenol by latero-vulvar route 7 days apart. After the second injection of d-cloprostenol, the ewes were checked for oestrus (every 12 hr) and then mated by fertile rams throughout the oestrous period. All ewes received 37.5 mu g of d-cloprostenol (latero-vulvar) and 1 mg of oestradiol benzoate by either intramuscular (EBim group; n = 12) or intravaginal (EBivg group; n = 11) route 16 hr before embryo flushing. Twenty minutes before the flushing, 50 IU of oxytocin were administered intravenously. The oestrous response (i.e., the percentage of ewes that showed signs of oestrous behaviour after the second d-cloprostenol injection) was 91.3% (21/23). The proportion of successfully penetrated ewes (81.8% compared with 80.0%), the mean duration of embryo flushing (24.7 +/- 2.0 min compared 26.2 +/- 1.9 min), the flushing fluid recovery rate (94.8 +/- 1.3% compared with 91.0 +/- 2.9%) and the average number of structures recovered per ewe (0.5 +/- 0.4 compared with 0.8 +/- 0.4) did not vary (p > 0.05) between the EBim and EBivg groups. Viable embryos were recovered from 41.2% (7/17) of successfully penetrated ewes. It can be concluded that nonsurgical (i.e., transcervical) embryo collection can be performed in oestrous-synchronized Santa Ines ewes pretreated with d-cloprostenol, oxytocin and oestradiol benzoate, with the latter hormone administered by either the intramuscular or intravaginal route.en
dc.description.affiliationEmbrapa Caprinos & Ovinos, Sobral, Brazil
dc.description.affiliationUniv Fed Vicosa, Vicosa, MG, Brazil
dc.description.affiliationEmbrapa Gado Leite, Juiz De Fora, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal, Brazil
dc.description.affiliationUniv Fed Fluminense, Niteroi, RJ, Brazil
dc.description.affiliationUniv Guelph, Guelph, ON, Canada
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal, Brazil
dc.description.sponsorshipEmbrapa Goats and Sheep
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdEmbrapa Goats and Sheep: 03.12.01.031.00.00
dc.description.sponsorshipIdFAPEMIG: PPM 00042-14
dc.description.sponsorshipIdCNPq: 310166/2012-8
dc.format.extent118-125
dc.identifierhttp://dx.doi.org/10.1111/rda.13318
dc.identifier.citationReproduction In Domestic Animals. Hoboken: Wiley, v. 54, n. 1, p. 118-125, 2019.
dc.identifier.doi10.1111/rda.13318
dc.identifier.issn0936-6768
dc.identifier.urihttp://hdl.handle.net/11449/184259
dc.identifier.wosWOS:000455228500014
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofReproduction In Domestic Animals
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcervical dilation
dc.subjectcloprostenol
dc.subjectoestradiol benzoate
dc.subjectoxytocin
dc.subjectsheep
dc.subjecttranscervical embryo collection
dc.titleCombined treatment with oestradiol benzoate, d-cloprostenol and oxytocin permits cervical dilation and nonsurgical embryo recovery in ewesen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell

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