Time-dependent expression of annexin 1 in a model of chronic granulomatous inflammation

dc.contributor.authorGibbs, L.
dc.contributor.authorCarollo, M. G.
dc.contributor.authorDamazo, A. S.
dc.contributor.authorOliani, S. M.
dc.contributor.authorPerretti, M.
dc.contributor.institutionWilliam Harvey Res Inst
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:00:21Z
dc.date.available2014-05-20T14:00:21Z
dc.date.issued2002-06-01
dc.description.abstractObjective and design: To determine the expression pattern and distribution of the glucocorticoid-inducible protein annexin 1 (ANXA1) in a murine model of chronic granulomatous inflammation.Materials or subjects: TO Mouse.Treatment: Chronic granulomatous inflammation was induced by injecting into dorsal sub-cutaneous air-pouches in mice, a mixture of croton oil and Freund's complete adjuvant (CO/FCA).Methods: Western and northern analysis, corticosterone assay, and immunohistochemistry. Statistical analysis was performed using ANOVA followed by Tukey's pair-wise comparisons or Dunnett's multiple comparisons.Results: ANXA1 protein levels changed significantly throughout the 4-week time course, with an initial peak at day 7 and a later elevation at 28 days. ANXA1 mRNA levels peaked at days 1 and 3, with a significant decline at day 7 followed by an upward trend to day 28. Plasma corticosterone measurements taken throughout the time course revealed an increase from 14 days onward, suggesting that corticosterone does not influence ANXA1 expression during the initial stages of the model. Immunogold staining revealed that ANXA1 expression in the inflamed tissue was mainly in extravasated neutrophils, with intact protein (37 kDa) being predominantly observed on the cell membrane.Conclusions: the pattern of ANXA1 expression indicates that infiltrated neutrophils are responsible for the majority of ANXA1 present both at early and later stages of this model of granulomatous inflammation.en
dc.description.affiliationWilliam Harvey Res Inst, Dept Biochem Pharmacol, London EC1M 6BQ, England
dc.description.affiliationUNESP, IBILCE, Dept Biol, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUNESP, IBILCE, Dept Biol, Sao Jose do Rio Preto, SP, Brazil
dc.format.extent300-306
dc.identifierhttp://dx.doi.org/10.1007/PL00000308
dc.identifier.citationInflammation Research. Basel: Birkhauser Verlag Ag, v. 51, n. 6, p. 300-306, 2002.
dc.identifier.doi10.1007/PL00000308
dc.identifier.issn1023-3830
dc.identifier.lattes5102737730539655
dc.identifier.urihttp://hdl.handle.net/11449/21350
dc.identifier.wosWOS:000176410300005
dc.language.isoeng
dc.publisherBirkhauser Verlag Ag
dc.relation.ispartofInflammation Research
dc.relation.ispartofjcr2.990
dc.relation.ispartofsjr1,062
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectLipocortin-1pt
dc.subjectneutrophilpt
dc.subjectair-pouchpt
dc.subjectmodelpt
dc.subjectgranulomapt
dc.titleTime-dependent expression of annexin 1 in a model of chronic granulomatous inflammationen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
dcterms.rightsHolderBirkhauser Verlag Ag
unesp.author.lattes5102737730539655
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
unesp.departmentBiologia - IBILCEpt

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