Hydroalcoholic crude extract of Casearia sylvestris Sw. reduces chronic post-ischemic pain by activation of pro-resolving pathways

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dc.contributor.authorPiovezan, Anna P.
dc.contributor.authorBatisti, Ana P.
dc.contributor.authorBenevides, Maria L.A.C.S.
dc.contributor.authorTurnes, Bruna L.
dc.contributor.authorMartins, Daniel F.
dc.contributor.authorKanis, Luiz
dc.contributor.authorDuarte, Elisa C.W.
dc.contributor.authorCavalheiro, Alberto J. [UNESP]
dc.contributor.authorBueno, Paula C.P. [UNESP]
dc.contributor.authorSeed, Michael P.
dc.contributor.authorNorling, Lucy V.
dc.contributor.authorCooper, Dianne
dc.contributor.authorHeadland, Sarah
dc.contributor.authorSouza, Patrícia R.P.S.
dc.contributor.authorPerretti, Mauro
dc.contributor.institutionPost-Graduate Programm in Health Science – Southern Univeristy of Santa Catarina (UNISUL)
dc.contributor.institutionLaboratory of Experimental Neuroscience (LANEX)– UNISUL
dc.contributor.institutionWilliam Harvey Research Institute – Queen Mary University of London/London
dc.contributor.institutionUndergraduation in Medicine – UNISUL
dc.contributor.institutionUniversidade Federal de Santa Catarina (UFSC)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:11:42Z
dc.date.available2018-12-11T17:11:42Z
dc.date.issued2017-05-23
dc.description.abstractEthnopharmacological relevance Casearia sylvestris Sw. is widely used in popular medicine to treat conditions associated with pain. Aim of the study The present study investigated the influence of hydroalcoholic crude extract of Casearia sylvestris (HCE-CS) and contribution of pro-resolving mediators on mechanical hyperalgesia in a mouse model of chronic post-ischemia pain (CPIP). Methods and results Male Swiss mice were subjected to ischemia of the right hind paw (3 h), then reperfusion was allowed. At 10 min, 24 h or 48 h post-ischemia/reperfusion (I/R), different groups of animals were treated with HCE-CS (30 mg/Kg, orally [p.o]), selected agonists at the pro-resolving receptor ALX/FPR2 (natural molecules like resolvin D1 and lipoxin A4 or the synthetic compound BML-111; 0.1–1 µg/animal) or vehicle (saline, 10 mL/Kg, s.c.), in the absence or presence of the antagonist WRW4 (10 µg, s.c.). Mechanical hyperalgesia (paw withdrawal to von Frey filament) was asseseed together with histological and immunostainning analyses. In these settings, pro-resolving mediators reduced mechanical hyperalgesia and HCE-CS or BML-111 displayed anti-hyperalgesic effects which was markedly attenuated in animals treated with WRW4. ALX/FPR2 expression was raised in skeletal muscle or neutrophils after treatment with HCE-CS or BML-111. Conclusion These results reveal significant antihyperalgesic effect of HCE-CS on CPIP, mediated at least in part, by the pathway of resolution of inflammation centred on the axis modulated by ALX/FPR2.en
dc.description.affiliationPost-Graduate Programm in Health Science – Southern Univeristy of Santa Catarina (UNISUL)
dc.description.affiliationLaboratory of Experimental Neuroscience (LANEX)– UNISUL
dc.description.affiliationWilliam Harvey Research Institute – Queen Mary University of London/London
dc.description.affiliationUndergraduation in Medicine – UNISUL
dc.description.affiliationLaboratory of Neurobiology of Pain and Inflammation – UFSC
dc.description.affiliationDepartment of Morphological Science – UFSC
dc.description.affiliationDepartment of Organic Chemistry/Institute of Chemistry – UNESP
dc.description.affiliationUnespDepartment of Organic Chemistry/Institute of Chemistry – UNESP
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 608765
dc.format.extent179-188
dc.identifierhttp://dx.doi.org/10.1016/j.jep.2017.03.059
dc.identifier.citationJournal of Ethnopharmacology, v. 204, p. 179-188.
dc.identifier.doi10.1016/j.jep.2017.03.059
dc.identifier.file2-s2.0-85019048828.pdf
dc.identifier.issn1872-7573
dc.identifier.issn0378-8741
dc.identifier.scopus2-s2.0-85019048828
dc.identifier.urihttp://hdl.handle.net/11449/174555
dc.language.isoeng
dc.relation.ispartofJournal of Ethnopharmacology
dc.relation.ispartofsjr1,150
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectALX/FPR2
dc.subjectCasearia sylvestris
dc.subjectChronic post-ischemia pain
dc.subjectInflammation
dc.subjectSalicaceae
dc.titleHydroalcoholic crude extract of Casearia sylvestris Sw. reduces chronic post-ischemic pain by activation of pro-resolving pathwaysen
dc.typeArtigo
unesp.author.lattes2518006820764120[8]
unesp.author.orcid0000-0001-8214-9957[8]

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