Genetic variability in species of bats revealed by RAPD analysis

Página do item simplificado
dc.contributor.authorMoreira, P. R. L. [UNESP]
dc.contributor.authorMorielle-Versute, E. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:22:03Z
dc.date.available2014-05-20T15:22:03Z
dc.date.issued2006-01-01
dc.description.abstractRandom amplified polymorphic DNA molecular marker was utilized as a means of analyzing genetic variability in seven bat species: Molossus molossus, M. rufus, Eumops glaucinus, E. perotis, Myotis nigricans, Eptesicus furinalis, and Artibeus planirostris. The determination of genetic diversity was based on 741 bands produced by a 20-random primer set. Only eight bands were considered monomorphic to one species. The greatest number of bands and the most polymorphic condition were exhibited by M. molossus, followed by M. nigricans, A. planirostris, E. furinalis, E. glaucinus, M. rufus, and E. perotis. Nei's genetic diversity index in the seven species considering the 20 primers was not greater than 0.22, but some primers were capable of detecting values between 0.39 and 0.49. Nei's unbiased genetic distance values and the UPGMA clustering pattern show that M. molossus and M. rufus have a close genetic relationship, unlike that observed between E. perotis and E. glaucinus. The latter was clustered with A. planirostris and E. furinalis. The low values for genetic diversity and distance observed indicate a genetic conservatism in the seven species. The fluorescent in situ hybridization experiments did not confirm a monomorphic condition for the eight bands identified, demonstrating that the monomorphic bands obtained by random amplified polymorphic DNA are insufficient for the identification of bat species.en
dc.description.affiliationUNESP, Inst Biociencias Letras & Ciências Exatas, Dept Zool & Bot, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUNESP, Inst Biociencias Letras & Ciências Exatas, Dept Biol, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias Letras & Ciências Exatas, Dept Zool & Bot, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias Letras & Ciências Exatas, Dept Biol, Sao Jose do Rio Preto, SP, Brazil
dc.format.extent804-815
dc.identifierhttp://www.geneticsmr.com/articles/314
dc.identifierhttp://www.geneticsmr.com//year2006/vol5-4/pdf/gmr0240.pdf
dc.identifier.citationGenetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 5, n. 4, p. 804-815, 2006.
dc.identifier.fileWOS000203012000020.pdf
dc.identifier.issn1676-5680
dc.identifier.scopus2-s2.0-39049190315
dc.identifier.urihttp://hdl.handle.net/11449/33120
dc.identifier.wosWOS:000203012000020
dc.language.isoeng
dc.publisherFunpec-editora
dc.relation.ispartofGenetics and Molecular Research
dc.relation.ispartofsjr0,439
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectChiropterapt
dc.subjectfluorescent in situ hybridizationpt
dc.subjectrandom amplified polymorphic DNApt
dc.subjectgenetic variationpt
dc.subjectBatspt
dc.titleGenetic variability in species of bats revealed by RAPD analysisen
dc.typeArtigo
dcterms.licensehttp://www.geneticsmr.com/node/5
dcterms.rightsHolderFunpec-editora
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000203012000020.pdf
Tamanho:
589.25 KB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Zoologia e Botânica - IBILCE
Artigos - Biologia - IBILCE