Human eosinophil adhesion and degranulation stimulated with eotaxin and RANTES in vitro: Lack of interaction with nitric oxide

dc.contributor.authorLintomen, Letícia
dc.contributor.authorFranchi, Gilberto
dc.contributor.authorNowill, Alexandre
dc.contributor.authorCondino-Neto, Antonio
dc.contributor.authorde Nucci, Gilberto
dc.contributor.authorZanesco, Angelina [UNESP]
dc.contributor.authorAntunes, Edson
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:23:38Z
dc.date.available2014-05-27T11:23:38Z
dc.date.issued2008-08-12
dc.description.abstractBackground: Airway eosinophilia is considered a central event in the pathogenesis of asthma. The toxic components of eosinophils are thought to be important in inducing bronchial mucosal injury and dysfunction. Previous studies have suggested an interaction between nitric oxide (NO) and chemokines in modulating eosinophil functions, but this is still conflicting. In the present study, we have carried out functional assays (adhesion and degranulation) and flow cytometry analysis of adhesion molecules (VLA-4 and Mac-1 expression) to evaluate the interactions between NO and CC-chemokines (eotaxin and RANTES) in human eosinophils. Methods: Eosinophils were purified using a percoll gradient followed byimmunomagnetic cell separator. Cell adhesion and degranulation were evaluated by measuring eosinophil peroxidase (EPO) activity, whereas expression of Mac-1 and VLA-4 was detected using flow cytometry. Results: At 4 h incubation, both eotaxin (100 ng/ml) and RANTES (1000 ng/ml) increased by 133% and 131% eosinophil adhesion, respectively. L-NAME alone (but not D-NAME) also increased the eosinophil adhesion, but the co-incubation of L-NAME with eotaxin or RANTES did not further affect the increased adhesion seen with chemokines alone. In addition, L-NAME alone (but not D-NAME) caused a significant cell degranulation, but it did not affect the CC-chemokine-induced cell degranulation. Incubation of eosinophils with eotaxin or RANTES, in absence or presence of L-NAME, did not affect the expression of VLA-4 and Mac-1 on eosinophil surface. Eotaxin and RANTES (100 ng/ml each) also failed to elevate the cyclic GMP levels above baseline in human eosinophils. Conclusion: Eotaxin and RANTES increase the eosinophil adhesion to fibronectin-coated plates and promote cell degranulation by NO-independent mechanisms. The failure of CC-chemokines to affect VLA-4 and Mac-1 expression suggests that changes in integrin function (avidity or affinity) are rather involved in the enhanced adhesion. © 2008 Lintomen et al; licensee BioMed Central Ltd.en
dc.description.affiliationDepartment of Pharmacology Faculty of Medical Sciences State University of Campinas (UNICAMP), Campinas, São Paulo
dc.description.affiliationDepartment of Physical Education Institute of Bioscience University of Sao Paulo State (UNESP), Rio Claro, SP
dc.description.affiliationUnespDepartment of Physical Education Institute of Bioscience University of Sao Paulo State (UNESP), Rio Claro, SP
dc.identifierhttp://dx.doi.org/10.1186/1471-2466-8-13
dc.identifier.citationBMC Pulmonary Medicine, v. 8.
dc.identifier.doi10.1186/1471-2466-8-13
dc.identifier.file2-s2.0-51249085806.pdf
dc.identifier.issn1471-2466
dc.identifier.lattes4472007237545596
dc.identifier.scopus2-s2.0-51249085806
dc.identifier.urihttp://hdl.handle.net/11449/70530
dc.language.isoeng
dc.relation.ispartofBMC Pulmonary Medicine
dc.relation.ispartofjcr2.721
dc.relation.ispartofsjr1,373
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCD11b antigen
dc.subjectcyclic GMP
dc.subjecteotaxin
dc.subjectfibronectin
dc.subjectintegrin
dc.subjectn(g) nitro dextro arginine methyl ester
dc.subjectn(g) nitroarginine methyl ester
dc.subjectnitric oxide
dc.subjectperoxidase
dc.subjectRANTES
dc.subjectvery late activation antigen 4
dc.subjectalpha4 integrin
dc.subjectbeta chemokine
dc.subjectenzyme inhibitor
dc.subjectcell stimulation
dc.subjectconcentration response
dc.subjectcontrolled study
dc.subjectdegranulation
dc.subjectenzyme activity
dc.subjecteosinophil
dc.subjectflow cytometry
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunomagnetic separation
dc.subjectin vitro study
dc.subjectincubation time
dc.subjectleukocyte adherence
dc.subjectleukocyte function
dc.subjectprotein analysis
dc.subjectprotein expression
dc.subjectprotein interaction
dc.subjectbiosynthesis
dc.subjectcell adhesion
dc.subjectcytology
dc.subjecteosinophilia
dc.subjectmetabolism
dc.subjectphysiology
dc.subjectAntigens, CD11b
dc.subjectCell Adhesion
dc.subjectCell Degranulation
dc.subjectChemokine CCL5
dc.subjectChemokines, CC
dc.subjectEnzyme Inhibitors
dc.subjectEosinophilia
dc.subjectEosinophils
dc.subjectFlow Cytometry
dc.subjectHumans
dc.subjectIntegrin alpha4
dc.subjectIntegrin alpha4beta1
dc.subjectMacrophage-1 Antigen
dc.subjectNG-Nitroarginine Methyl Ester
dc.subjectNitric Oxide
dc.titleHuman eosinophil adhesion and degranulation stimulated with eotaxin and RANTES in vitro: Lack of interaction with nitric oxideen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
unesp.author.lattes4472007237545596
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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