A Short-Term High-Fat Diet Alters Glutathione Levels and IL-6 Gene Expression in Oxidative Skeletal Muscles of Young Rats

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dc.contributor.authorAndrich, David E.
dc.contributor.authorMelbouci, Lilya
dc.contributor.authorOu, Ya
dc.contributor.authorAuclair, Nickolas
dc.contributor.authorMercier, Jocelyne
dc.contributor.authorGrenier, Jean-Christophe
dc.contributor.authorLira, Fabio Santos [UNESP]
dc.contributor.authorBarreiro, Luis B.
dc.contributor.authorDanialou, Gawiyou
dc.contributor.authorCorntois, Alain-Steve
dc.contributor.authorLavoie, Jean-Claude
dc.contributor.authorSt-Pierre, David H.
dc.contributor.institutionUniv Quebec Montreal UQAM
dc.contributor.institutionCHU St Justine
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Montreal
dc.contributor.institutionRoyal Mil Coll St Jean
dc.date.accessioned2019-10-04T12:13:40Z
dc.date.available2019-10-04T12:13:40Z
dc.date.issued2019-04-10
dc.description.abstractObesity and ensuing disorders are increasingly prevalent worldwide. High-fat diets (HFD) and diet-induced obesity have been shown to induce oxidative stress and inflammation while altering metabolic homeostasis in many organs, including the skeletal muscle. We previously observed that 14 days of HFD impairs contractile functions of the soleus (SOL) oxidative skeletal muscle. However, the mechanisms underlying these effects are not clarified. In order to determine the effects of a short-term HFD on skeletal muscle glutathione metabolism, young male Wistar rats (100-125 g) were fed HFD or a regular chow diet (RCD) for 14 days. Reduced (GSH) and disulfide (GSSG) glutathione levels were measured in the SOL. The expression of genes involved in the regulation of glutathione metabolism, oxidative stress, antioxidant defense and inflammation were measured by RNA-Seq. We observed a significant 25% decrease of GSH levels in the SOL muscle. Levels of GSSG and the GSH:GSSG ratio were similar in both groups. Further, we observed a 4.5 fold increase in the expression of pro-inflammatory cytokine interleukin 6 (IL-6) but not of other cytokines or markers of inflammation and oxidative stress. We hereby demonstrate that a short-term HFD significantly lowers SOL muscle GSH levels. This effect could be mediated through the increased expression of IL-6. Further, the skeletal muscle antioxidant defense could be impaired under cellular stress. We surmise that these early alterations could contribute to HFD-induced insulin resistance observed in longer protocols.en
dc.description.affiliationUniv Quebec Montreal UQAM, Dept Sci & Act Phys, Montreal, PQ, Canada
dc.description.affiliationUniv Quebec Montreal UQAM, GRAPA, Montreal, PQ, Canada
dc.description.affiliationUniv Quebec Montreal UQAM, Dept Sci Biol, Montreal, PQ, Canada
dc.description.affiliationCHU St Justine, Ctr Rech, Montreal, PQ, Canada
dc.description.affiliationSao Paulo State Univ, Dept Phys Educ, Sao Paulo, Brazil
dc.description.affiliationUniv Montreal, Fac Med, Dept Pediat, Montreal, PQ, Canada
dc.description.affiliationRoyal Mil Coll St Jean, St Jean, PQ, Canada
dc.description.affiliationUniv Montreal, Fac Med, Dept Nutr, Montreal, PQ, Canada
dc.description.affiliationUnespSao Paulo State Univ, Dept Phys Educ, Sao Paulo, Brazil
dc.description.sponsorshipNSERC
dc.description.sponsorshipFQRS Chercheur Boursier Junior 1 Award
dc.description.sponsorshipIdNSERC: 418509-2012
dc.format.extent10
dc.identifierhttp://dx.doi.org/10.3389/fphys.2019.00372
dc.identifier.citationFrontiers In Physiology. Lausanne: Frontiers Media Sa, v. 10, 10 p., 2019.
dc.identifier.doi10.3389/fphys.2019.00372
dc.identifier.issn1664-042X
dc.identifier.urihttp://hdl.handle.net/11449/184444
dc.identifier.wosWOS:000464457800001
dc.language.isoeng
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers In Physiology
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjecthigh-fat diet
dc.subjectyoung rats
dc.subjectmuscle glutathione
dc.subjectoxidative stress
dc.subjectinflammation
dc.subjectgene expression
dc.titleA Short-Term High-Fat Diet Alters Glutathione Levels and IL-6 Gene Expression in Oxidative Skeletal Muscles of Young Ratsen
dc.typeArtigo
dcterms.rightsHolderFrontiers Media Sa
unesp.author.orcid0000-0002-6068-5801[1]
unesp.author.orcid0000-0001-7937-5160[6]

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